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  Ambient Mass Spectrometry Imaging with Picosecond Infrared Laser Ablation Electrospray Ionization (PIR-LAESI)

Zou, J., Talbot, F., Tata, A., Ermini, L., Franjic, K., Ventura, M., et al. (2015). Ambient Mass Spectrometry Imaging with Picosecond Infrared Laser Ablation Electrospray Ionization (PIR-LAESI). Analytical Chemistry, 87(24), 12071-12079. doi:10.1021/acs.analchem.5b02756.

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http://dx.doi.org/10.1021/acs.analchem.5b02756 (Publisher version)
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 Creators:
Zou, Jing1, Author
Talbot, Francis2, Author
Tata, Alessandra3, 4, Author
Ermini, Leonardo5, Author
Franjic, Kresimir1, Author
Ventura, Manuela4, Author
Zheng, Jinzi4, Author
Ginsberg, Howard6, 7, Author
Post, Martin5, 8, Author
Ifa, Demian R.3, Author
Jaffray, David4, 9, Author
Miller, R. J. Dwayne1, 2, 10, Author           
Zarrine-Afsar, Arash4, 6, 7, 9, Author
Affiliations:
1Department of Physics, University of Toronto, 60 St George Street, Toronto, Ontario M5S 1A7, Canada, ou_persistent22              
2Department of Chemistry, University of Toronto, 80 St George Street, Toronto, Ontario M5S 3H6, Canada, ou_persistent22              
3Department of Chemistry, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3, Canada, ou_persistent22              
4Techna Institute for the Advancement of Technology for Health, University Health Network, Toronto, Ontario M5G 1P5, Canada, ou_persistent22              
5Program in Physiology and Experimental Medicine, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada, ou_persistent22              
6Department of Surgery, University of Toronto, 149 College Street, Toronto, Ontario M5T 1P5, Canada, ou_persistent22              
7Keenan Research Centre for Biomedical Science, Li Ka Shing Knowledge Institute, St. Michael’s Hospital, 30 Bond Street, Toronto, Ontario M5B 1W8, Canada, ou_persistent22              
8Institute of Medical Science, University of Toronto, Toronto, Ontario M5S 1A8, Canada, ou_persistent22              
9Department of Medical Biophysics, University of Toronto, 101 College Street Suite 15-701, Toronto, Ontario M5G 1L7, Canada, ou_persistent22              
10Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              

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 Abstract: A picosecond infrared laser (PIRL) is capable of cutting through biological tissues in the absence of significant thermal damage. As such, PIRL is a standalone surgical scalpel with the added bonus of minimal postoperative scar tissue formation. In this work, a tandem of PIRL ablation with electrospray ionization (PIR-LAESI) mass spectrometry is demonstrated and characterized for tissue molecular imaging, with a limit of detection in the range of 100 nM for reserpine or better than 5 nM for verapamil in aqueous solution. We characterized PIRL crater size using agar films containing Rhodamine. PIR-LAESI offers a 20–30 μm vertical resolution (∼3 μm removal per pulse) and a lateral resolution of ∼100 μm. We were able to detect 25 fmol of Rhodamine in agar ablation experiments. PIR-LAESI was used to map the distribution of endogenous methoxykaempferol glucoronide in zebra plant (Aphelandra squarrosa) leaves producing a localization map that is corroborated by the literature. PIR-LAESI was further used to image the distribution inside mouse kidneys of gadoteridol, an exogenous magnetic resonance contrast agent intravenously injected. Parallel mass spectrometry imaging (MSI) using desorption electrospray ionization (DESI) and matrix assisted laser desorption ionization (MALDI) were performed to corroborate PIR-LAESI images of the exogenous agent. We further show that PIR-LAESI is capable of desorption ionization of proteins as well as phospholipids. This comparative study illustrates that PIR-LAESI is an ion source for ambient mass spectrometry applications. As such, a future PIRL scalpel combined with secondary ionization such as ESI and mass spectrometry has the potential to provide molecular feedback to guide PIRL surgery.

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Language(s): eng - English
 Dates: 2015-07-222015-11-112015-11-112015-12-15
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acs.analchem.5b02756
 Degree: -

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Title: Analytical Chemistry
  Abbreviation : Anal. Chem.
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 87 (24) Sequence Number: - Start / End Page: 12071 - 12079 Identifier: Other: 0003-2700
CoNE: https://pure.mpg.de/cone/journals/resource/111032812862552