English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Interaction of the v-and c-Myb proteins with regulatory sequences of the human c-myc gene.

Zobel, A., Kalkbrenner, F., Guehmann, S., Nawrath, M., Vorbrüggen, G., & Mölling, K. (1991). Interaction of the v-and c-Myb proteins with regulatory sequences of the human c-myc gene. Oncogene, 6(8), 1397-1407.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-2930-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-2931-7
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Zobel, A., Author
Kalkbrenner, F., Author
Guehmann, S., Author
Nawrath, M., Author
Vorbrüggen, G.1, Author              
Mölling, K., Author
Affiliations:
1Research Group of Molecular Cell Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578593              

Content

show
hide
Free keywords: -
 Abstract: Eight c-Myb-binding sites have been identified in the regulatory region of the human c-myc gene using gel retardation and DNAase I footprint assays with purified bacterially expressed full-length and carboxy-terminally truncated c-Myb proteins. These binding sites exhibit different affinities whereby strong binding correlates better with conservation of the palindromic sequences, AACXGTT or AACGTT, than the previously described consensus sequence. Flanking AT-rich sequences further increase the binding affinity. The c-Myb-binding sites are arranged in pairs consisting of one high- and one low-affinity binding site. Binding of the Myb proteins to these sites is non-cooperative. The v-Myb protein protects two nucleotides fewer than the c-Myb protein. Co-transfection of reporter CAT genes, containing upstream human c-myc sequences including exon 1, with c-Myb-expressing constructs resulted in positive transactivation, which was eightfold with full-length Myb and 14-fold with the truncated Myb. This result suggests that the Myb protein could participate in regulation of human c-myc gene expression.

Details

show
hide
Language(s): eng - English
 Dates: 1991-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Oncogene
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 6 (8) Sequence Number: - Start / End Page: 1397 - 1407 Identifier: -