English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Plasmodium apicoplast Gln-tRNAGln biosynthesis utilizes a unique GatAB amidotransferase essential for erythrocytic stage parasites

Mailu, B. M., Li, L., Arthur, J., Nelson, T. M., Ramasamy, G., Fritz-Wolf, K., et al. (2015). Plasmodium apicoplast Gln-tRNAGln biosynthesis utilizes a unique GatAB amidotransferase essential for erythrocytic stage parasites. The Journal of Biological Chemistry, 290(49), 29629-29641. doi:10.1074/jbc.M115.655100.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-2984-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-2985-B
Genre: Journal Article
Other : P. falciparum apicoplast glutamyl-tRNA amidotransferase

Files

show Files
hide Files
:
JBiolChem_epub_2015_100.pdf (Any fulltext), 4MB
 
File Permalink:
-
Name:
JBiolChem_epub_2015_100.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Description:
-

Creators

show
hide
 Creators:
Mailu, Boniface M., Author
Li, Ling, Author
Arthur, Jen, Author
Nelson, Todd M., Author
Ramasamy, Gowthaman, Author
Fritz-Wolf, Karin1, Author              
Becker, Katja, Author
Gardner, Malcolm J., Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Content

show
hide
Free keywords: apicoplast; glutamyl-tRNA amidotransferase; glutamyl-tRNA synthetase; malaria; nucleic acid; nucleic acid enzymology; plasmodium; transfer RNA (tRNA)
 Abstract: The malaria parasite Plasmodium falciparum apicoplast indirect aminoacylation pathway utilizes a non-discriminating glutamyl-tRNA synthetase to synthesize Glu-tRNA(Gln) and a glutaminyl-tRNA amidotransferase to convert Glu-tRNA(Gln) to Gln-tRNA(Gln). Here, we show that Plasmodium falciparum and other apicomplexans possess a unique heterodimeric glutamyl-tRNA amidotransferase consisting of GatA and GatB subunits (GatAB). We localized the P. falciparum GatA and GatB subunits to the apicoplast in blood stage parasites and demonstrated that recombinant GatAB converts Glu-tRNA(Gln) to Gln-tRNA(Gln) in vitro. We demonstrate that the apicoplast GatAB-catalyzed reaction is essential to the parasite blood stages because we could not delete the Plasmodium berghei gene encoding GatA in blood stage parasites in vivo. A phylogenetic analysis placed the split between Plasmodium GatB, archaeal GatE, and bacterial GatB prior to the phylogenetic divide between bacteria and archaea. Moreover, Plasmodium GatA also appears to have emerged prior to the bacterial-archaeal phylogenetic divide. Thus, although GatAB is found in Plasmodium, it emerged prior to the phylogenetic separation of archaea and bacteria.

Details

show
hide
Language(s): eng - English
 Dates: 2015-08-132015-03-272015-08-282015-12-04
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Journal of Biological Chemistry
  Other : JBC
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Pages: - Volume / Issue: 290 (49) Sequence Number: - Start / End Page: 29629 - 29641 Identifier: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826_1