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  Real-time label-free direct electronic monitoring of topoisomerase enzyme binding kinetics on graphene.

Zuccaro, L., Tesauro, C., Kurkina, T., Fiorani, P., Yu, H. K., Knudsen, B. R., et al. (2015). Real-time label-free direct electronic monitoring of topoisomerase enzyme binding kinetics on graphene. ACS Nano, 9(11), 11166-11176. doi:10.1021/acsnano.5b05709.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-3312-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-6171-B
Genre: Journal Article

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 Creators:
Zuccaro, L., Author
Tesauro, C., Author
Kurkina, T., Author
Fiorani, P., Author
Yu, H. K.1, Author              
Knudsen, B. R., Author
Kern, K., Author
Desideri, A., Author
Balasubramanian, K., Author
Affiliations:
1Department of Dynamics at Surfaces, MPI for Biophysical Chemistry, Max Planck Society, ou_578600              

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Free keywords: label-free biosensing; human topoisomerase; enzyme activity; biosensor; field effect
 Abstract: Monolayer graphene field-effect sensors operating in liquid have been widely deployed for detecting a range of analyte species often under equilibrium conditions. Here we report on the real-time detection of the binding kinetics of the essential human enzyme, topoisomerase I interacting with substrate molecules (DNA probes) that are immobilized electrochemically on to monolayer graphene strips. By monitoring the field-effect characteristics of the graphene biosensor in real-time during the enzyme–substrate interactions, we are able to decipher the surface binding constant for the cleavage reaction step of topoisomerase I activity in a label-free manner. Moreover, an appropriate design of the capture probes allows us to distinctly follow the cleavage step of topoisomerase I functioning in real-time down to picomolar concentrations. The presented results are promising for future rapid screening of drugs that are being evaluated for regulating enzyme activity.

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Language(s): eng - English
 Dates: 2015-10-072015-11-24
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1021/acsnano.5b05709
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Title: ACS Nano
Source Genre: Journal
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Pages: - Volume / Issue: 9 (11) Sequence Number: - Start / End Page: 11166 - 11176 Identifier: -