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  Structure of GPN-loop GTPase Npa3 and implications for RNA polymerase II assembly.

Niesser, J., Wagner, F. R., Kostrewa, D., Mühlbacher, W., & Cramer, P. (2016). Structure of GPN-loop GTPase Npa3 and implications for RNA polymerase II assembly. Molecular and Cellular Biology, 36(5), 820-831. doi:10.1128/MCB.01009-15.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-4273-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-7C53-B
Genre: Journal Article

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Niesser, J.1, Author              
Wagner, F. R., Author
Kostrewa, D., Author
Mühlbacher, W.1, Author              
Cramer, P.1, Author              
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1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: Biogenesis of the 12-subunit RNA polymerase II (Pol II) transcription complex requires so-called GPN-loop GTPases, but the function of these enzymes is unknown. Here we report the first crystal structure of a eukaryotic GPN-loop GTPase, the S. cerevisiae enzyme Npa3 (a homolog of human GPN1, also called RPAP4, XAB1, MBDin), and analyze its catalytic mechanism. The enzyme was trapped in a GDP-bound, closed conformation, and in a novel GTP analogue-bound, open conformation displaying a conserved hydrophobic pocket distant from the active site. We show that Npa3 has chaperone activity and interacts with hydrophobic peptide regions of Pol II subunits that form interfaces in the assembled Pol II complex. Biochemical results are consistent with a model that the hydrophobic pocket binds peptides, and that this can allosterically stimulate GTPase activity and subsequent peptide release. These results suggest that GPN-loop GTPases are assembly chaperones for Pol II and other protein complexes.

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Language(s): eng - English
 Dates: 2015-12-282016-03
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1128/MCB.01009-15
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Title: Molecular and Cellular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 36 (5) Sequence Number: - Start / End Page: 820 - 831 Identifier: -