English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  GPI-anchored proteins do not reside in ordered domains in the live cell plasma membrane.

Sevcsik, E., Brameshuber, M., Fölser, M., Weghuber, J., Honigmann, A., & Schütz, G. J. (2015). GPI-anchored proteins do not reside in ordered domains in the live cell plasma membrane. Biophysical Journal, 108(Supplement 1), 32a-33a.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-45CD-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-45D7-6
Genre: Conference Paper

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Sevcsik, E., Author
Brameshuber, M., Author
Fölser, M., Author
Weghuber, J., Author
Honigmann, A.1, Author              
Schütz, G. J., Author
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

Content

show
hide
Free keywords: -
 Abstract: The organization of proteins and lipids in the plasma membrane has been the subject of a long-lasting debate. Membrane rafts of higher lipid chain order were proposed to mediate protein interactions, but have thus far not been directly observed. Here we use protein micropatterning combined with single-molecule tracking to put current models to the test: we rearranged lipid-anchored raft proteins (glycosylphosphatidylinositol(GPI)-anchored-mGFP) directly in the live cell plasma membrane and measured the effect on the local membrane environment. Intriguingly, this treatment does neither nucleate the formation of an ordered membrane phase nor result in any enrichment of nanoscopic-ordered domains within the micropatterned regions. In contrast, we find that immobilized mGFP-GPIs behave as inert obstacles to the diffusion of other membrane constituents without influencing their membrane environment over distances beyond their physical size. Our results indicate that phase partitioning is not a fundamental element of protein organization in the plasma membrane.

Details

show
hide
Language(s): eng - English
 Dates: 2015-01-27
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: -
 Degree: -

Event

show
hide
Title: 59th Annual Meeting of the Biophysical-Society
Place of Event: Baltimore/MD
Start-/End Date: 2015-02-07 - 2015-02-11

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biophysical Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 108 (Supplement 1) Sequence Number: - Start / End Page: 32a - 33a Identifier: -