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  Cell type- and brain region-resolved mouse brain proteome

Sharma, K., Schmitt, S., Bergner, C. G., Tyanova, S., Kannaiyan, N., Manrique-Hoyos, N., et al. (2015). Cell type- and brain region-resolved mouse brain proteome. NATURE NEUROSCIENCE, 18(12), 1819-1831. doi:10.1038/nn.4160.

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 Creators:
Sharma, Kirti1, Author              
Schmitt, Sebastian2, Author
Bergner, Caroline G.2, Author
Tyanova, Stefka1, Author              
Kannaiyan, Nirmal2, Author
Manrique-Hoyos, Natalia2, Author
Kongi, Karina2, Author
Cantuti, Ludovico2, Author
Hanisch, Uwe-Karsten2, Author
Philips, Mari-Anne2, Author
Rossner, Moritz J.2, Author
Mann, Matthias1, Author              
Simons, Mikael2, Author
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2external, ou_persistent22              

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Free keywords: TRANSLATIONAL PROFILING APPROACH; MOLECULAR CHARACTERIZATION; HETEROPHILIC INTERACTIONS; NEURITE OUTGROWTH; ADHESION MOLECULE; MEMBRANE-PROTEIN; GENE-EXPRESSION; TRANSCRIPTOME; MYELINATION; NEUROTRIMIN
 Abstract: Brain transcriptome and connectome maps are being generated, but an equivalent effort on the proteome is currently lacking. We performed high-resolution mass spectrometry based proteomics for in-depth analysis of the mouse brain and its major brain regions and cell types. Comparisons of the 12,934 identified proteins in oligodendrocytes, astrocytes, microglia and cortical neurons with deep sequencing data of the transcriptome indicated deep coverage of the proteome. Cell type specific proteins defined as tenfold more abundant than average expression represented about a tenth of the proteome, with an overrepresentation of cell surface proteins. To demonstrate the utility of our resource, we focused on this class of proteins and identified Lsamp, an adhesion molecule of the IgLON family, as a negative regulator of myelination. Our findings provide a framework for a system-level understanding of cell-type diversity in the CNS and serves as a rich resource for analyses of brain development and function.

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Language(s): eng - English
 Dates: 2015
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000365458600021
DOI: 10.1038/nn.4160
 Degree: -

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Title: NATURE NEUROSCIENCE
Source Genre: Journal
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Publ. Info: 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 18 (12) Sequence Number: - Start / End Page: 1819 - 1831 Identifier: ISSN: 1097-6256