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  Crystal structure of the motor domain of a class-I myosin

Kollmar, M., Dürrwang, U., Kliche, W., Manstein, D. J., & Kull, F. J. (2002). Crystal structure of the motor domain of a class-I myosin. EMBO Journal, 21(11), 2517-2525. doi:10.1093/emboj/21.11.2517.

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Genre: Journal Article
Alternative Title : Crystal structure of the motor domain of a class-I myosin

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EMBOJ_21_2002_2517.pdf (Any fulltext), 305KB
 
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 Creators:
Kollmar, Martin1, Author              
Dürrwang, Ulrike1, Author              
Kliche, Werner2, Author              
Manstein, Dietmar J.1, Author              
Kull, F. Jon1, Author              
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1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
2Emeritus Group Bioorganic Chemistry, Max Planck Institute for Medical Research, Max Planck Society, ou_1497711              

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Free keywords: crystal structure; Dictyostelium discoideum; motor protein; myosin-I; unconventional myosin
 Abstract: The crystal structure of the motor domain of Dictyostelium discoideum myosin‐IE, a monomeric unconventional myosin, was determined. The crystallographic asymmetric unit contains four independently resolved molecules, highlighting regions that undergo large conformational changes. Differences are particularly pronounced in the actin binding region and the converter domain. The changes in position of the converter domain reflect movements both parallel to and perpendicular to the actin axis. The orientation of the converter domain is ∼30° further up than in other myosin structures, indicating that MyoE can produce a larger power stroke by rotating its lever arm through a larger angle. The role of extended loops near the actin‐binding site is discussed in the context of cellular localization. The core regions of the motor domain are similar, and the structure reveals how that core is stabilized in the absence of an N‐terminal SH3‐like domain.

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Language(s): eng - English
 Dates: 2002-03-112001-11-162002-04-052002-06-032002-06-03
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: EMBO Journal
  Other : EMBO J.
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 21 (11) Sequence Number: - Start / End Page: 2517 - 2525 Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061