ausblenden:
Schlagwörter:
MUCINOUS HISTOLOGY PREDICTS; MALIGNANT MELANOMA-CELL; COLON-CANCER;
ANTIGEN P97; QUANTITATIVE PROTEOMICS; TUMOR-ANTIGEN; ADENOMA; PROTEIN;
IDENTIFICATION; TRANSFERRINMelanotransferrin; colorectal carcinoma; poorly differentiated
adenocarcinoma; pathogenesis; adenoma-carcinoma sequence; tumorigenesis;
Zusammenfassung:
Background: Our previous liquid chromatographytandem mass spectrometry (LC-MS/MS) study on colorectal cancer proteome resulted in identification of 10,000 differentially expressed proteins. We observed a significantly changed expression of 25% of all identified proteins between patient and matched adjacent normal mucosa, carcinoma and colorectal adenoma, including melanotransferrin. Herein, we consider this protein as a potential biomarker of colorectal cancer. Materials and Methods: Immunohistochemical detection of melanotransferrin was carried-out to localize its expression pattern within the colorectal tissues by tissue microarray. The diagnostic utility of melanotransferrin was evaluated in patient serum by enzyme-linked immunosorbent assay (ELISA). Results: Strong melanotransferrin expression was found to be related to clinicopathological characteristics, lymph node involvement (p=0.008), tumor localization in colon (p=0.001), presence of mucin (p<0.013) and increasing tumor grade (p<0.001). Melanotransferrin level in serum from patients with colorectal cancer was significantly higher than that in healthy controls (p<0.001). Conclusion: We provide novel evidence that melanotransferrin may be involved in transformation from benign tumor to malignancy and is a marker of an invasive tumor phenotype.
