ausblenden:
Schlagwörter:
FLUORESCENCE CORRELATION SPECTROSCOPY; GIANT UNILAMELLAR VESICLES;
IMMUNE-SYSTEM; PHOSPHOLIPID-BILAYERS; MEMBRANE DYNAMICS; CELL-ADHESION;
ANNEXIN-V; APOPTOSIS; RECEPTOR; DIFFUSIONFluorescence correlation spectroscopy; Supported lipid bilayer;
Apoptosis; Functionalized surface; Fas receptor;
Zusammenfassung:
Extrinsic apoptosis is initiated by recognition and clustering of the single-pass transmembrane proteins Fas ligand and Fas expressed at the surface of closely apposed lymphocytes and target cells, respectively. Since Fas-mediated death response was mainly studied with soluble antibodies, the mobility constraints for receptor activation by a membrane embedded agonist is not well understood. We explored this influence by stimulating apoptosis on functionalized supported lipid bilayers, where we quantified agonist mobility by z-scan fluorescence correlation spectroscopy. Using different lipid compositions, we show that the apoptotic response correlates with increased lateral mobility of the agonist in the lipid bilayer. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.