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  A mini-twister variant and impact of residues/cations on the phosphodiester cleavage of this ribozyme class.

Kosutic, M., Neuner, S., Ren, A., Flür, S., Wunderlich, C., Mairhofer, E., et al. (2015). A mini-twister variant and impact of residues/cations on the phosphodiester cleavage of this ribozyme class. Angewandte Chemie International Edition, 54(50), 15128-15133. doi:10.1002/anie.201506601.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-713B-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-1429-6
Genre: Journal Article

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 Creators:
Kosutic, M., Author
Neuner, S., Author
Ren, A., Author
Flür, S., Author
Wunderlich, C., Author
Mairhofer, E., Author
Vusurovic, N., Author
Seikowski, J.1, Author              
Breuker, K., Author
Höbartner, C.1, Author              
Patel, D. J., Author
Kreutz, C., Author
Micura, R., Author
Affiliations:
1Research Group of Nucleic Acid Chemistry, MPI for Biophysical Chemistry, Max Planck Society, ou_578605              

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 Abstract: Nucleolytic ribozymes catalyze site-specific cleavage of their phosphodiester backbones. A minimal version of the twister ribozyme is reported that lacks the phylogenetically conserved stem P1 while retaining wild-type activity. Atomic mutagenesis revealed that nitrogen atoms N1 and N3 of the adenine-6 at the cleavage site are indispensable for cleavage. By NMR spectroscopy, a pKa value of 5.1 was determined for a 13C2-labeled adenine at this position in the twister ribozyme, which is significantly shifted compared to the pKa of the same adenine in the substrate alone. This finding pinpoints at a potential role for adenine-6 in the catalytic mechanism besides the previously identified invariant guanine-48 and a Mg2+ ion, both of which are directly coordinated to the non-bridging oxygen atoms of the scissile phosphate; for the latter, additional evidence stems from the observation that Mn2+ or Cd2+ accelerated cleavage of phosphorothioate substrates. The relevance of this metal ion binding site is further emphasized by a new 2.6 Å X-ray structure of a 2′-OCH3-U5 modified twister ribozyme.

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Language(s): eng - English
 Dates: 2015-10-162015-12-07
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1002/anie.201506601
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Title: Angewandte Chemie International Edition
Source Genre: Journal
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Pages: - Volume / Issue: 54 (50) Sequence Number: - Start / End Page: 15128 - 15133 Identifier: -