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  In vivo, low-resistance, whole-cell recordings from neurons in the anaesthetized and awake mammalian brain

Margrie, T. W., Brecht, M., & Sakmann, B. (2002). In vivo, low-resistance, whole-cell recordings from neurons in the anaesthetized and awake mammalian brain. Pflügers Archiv: European Journal of Physiology, 444(4), 491-498. doi:10.1007/s00424-002-0831-z.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-71D3-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-71D4-E
Genre: Journal Article
Alternative Title : In vivo, low-resistance, whole-cell recordings from neurons in the anaesthetized and awake mammalian brain

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PflügArch_444_2002_491.pdf (Any fulltext), 114KB
 
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 Creators:
Margrie, Troy W.1, 2, Author              
Brecht, Michael1, Author              
Sakmann, Bert1, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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Free keywords: In vivo; Whole-cell; Olfactory bulb; Barrel cortex; Evoked; Spontaneous; Awake; Anaesthetized
 Abstract: A blind patch-clamp technique for in vivo whole-cell recordings in the intact brain is described. Recordings were obtained from various neuronal cell types located 100–5,000 µm from the cortical surface. Access resistance of recordings was as low as 10 MΩ but increased with recording depth and animal age. Recordings were remarkably stable and it was therefore possible to obtain whole-cell recordings in awake, head-fixed animals. The whole-cell configuration permitted rapid dialysis of cells with a calcium buffer. In most neurons very little ongoing action potential (AP) activity was observed and the spontaneous firing rates were up to 50-fold less than what has been reported by extracellular unit recordings. AP firing in the brain might therefore be far sparser than previously thought.

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Language(s): eng - English
 Dates: 2002-01-092002-02-192002-07
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Pflügers Archiv: European Journal of Physiology
  Other : Pflügers Arch. Europ. J. Physiol.
Source Genre: Journal
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Publ. Info: Heidelberg : Springer-Verlag
Pages: - Volume / Issue: 444 (4) Sequence Number: - Start / End Page: 491 - 498 Identifier: ISSN: 0031-6768
CoNE: https://pure.mpg.de/cone/journals/resource/954925432380