hide
Free keywords:
In vivo; Whole-cell; Olfactory bulb; Barrel cortex; Evoked; Spontaneous; Awake; Anaesthetized
Abstract:
A blind patch-clamp technique for in vivo whole-cell recordings in the intact brain is described. Recordings were obtained from various neuronal cell types located 100–5,000 µm from the cortical surface. Access resistance of recordings was as low as 10 MΩ but increased with recording depth and animal age. Recordings were remarkably stable and it was therefore possible to obtain whole-cell recordings in awake, head-fixed animals. The whole-cell configuration permitted rapid dialysis of cells with a calcium buffer. In most neurons very little ongoing action potential (AP) activity was observed and the spontaneous firing rates were up to 50-fold less than what has been reported by extracellular unit recordings. AP firing in the brain might therefore be far sparser than previously thought.
