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  Morpholgy and dynamcis of the endocytic pathway in Dictyostelium discoideum

Neuhaus, E. M., Almers, W., & Soldati, T. (2002). Morpholgy and dynamcis of the endocytic pathway in Dictyostelium discoideum. Molecular and Cellular Biology (Washington, DC), 13(4), 1390-1407. doi:10.1091/mbc.01-08-0392.

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Genre: Journal Article
Alternative Title : Morpholgy and dynamcis of the endocytic pathway in Dictyostelium discoideum

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MolBiolCell_13_2002_1390.pdf (Any fulltext), 2MB
 
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Neuhaus, Eva Maria1, Author              
Almers, Wolfhard1, Author              
Soldati, Thierry1, Author              
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1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

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 Abstract: Dictyostelium discoideum is a genetically and biochemically tractable social amoeba belonging to the crown group of eukaryotes. It performs some of the tasks characteristic of a leukocyte such as chemotactic motility, macropinocytosis, and phagocytosis that are not performed by other model organisms or are difficult to study.D. discoideum is becoming a popular system to study molecular mechanisms of endocytosis, but the morphological characterization of the organelles along this pathway and the comparison with equivalent and/or different organelles in animal cells and yeasts were lagging. Herein, we used a combination of evanescent wave microscopy and electron microscopy of rapidly frozen samples to visualize primary endocytic vesicles, vesicular-tubular structures of the early and late endo-lysosomal system, such as multivesicular bodies, and the specialized secretory lysosomes. In addition, we present biochemical and morphological evidence for the existence of a micropinocytic pathway, which contributes to the uptake of membrane along side macropinocytosis, which is the major fluid phase uptake process. This complex endosomal compartment underwent continuous cycles of tubulation/vesiculation as well as homo- and heterotypic fusions, in a way reminiscent of mechanisms and structures documented in leukocytes. Finally, egestion of fluid phase from the secretory lysosomes was directly observed.

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Language(s): eng - English
 Dates: 2001-12-272001-08-082002-01-182002-02-222002-04
 Publication Status: Published in print
 Pages: 18
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 Rev. Type: Peer
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Title: Molecular and Cellular Biology (Washington, DC)
  Other : Mol Cell Biol
Source Genre: Journal
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Publ. Info: American Society for Microbiology (ASM)
Pages: - Volume / Issue: 13 (4) Sequence Number: - Start / End Page: 1390 - 1407 Identifier: ISSN: 0270-7306
CoNE: https://pure.mpg.de/cone/journals/resource/954925502188