日本語
 
Help Privacy Policy ポリシー/免責事項
  詳細検索ブラウズ

アイテム詳細

  Orientation and protein-cofactor interactions of monosubstituted n-alkyl naphthoquinones in the A1 binding site of photosystem I.

Pushkar, Y. N., Zech, S. G., Stehlik, D., Brown, S., van der Est, A., & Zimmermann, H. (2002). Orientation and protein-cofactor interactions of monosubstituted n-alkyl naphthoquinones in the A1 binding site of photosystem I. The Journal of Physical Chemistry B, 106(46), 12052-12058. doi:10.1021/jp0265743.

Item is

基本情報

表示: 非表示:
資料種別: 学術論文
その他のタイトル : Orientation and protein-cofactor interactions of monosubstituted n-alkyl naphthoquinones in the A1 binding site of photosystem I.

ファイル

表示: ファイル
非表示: ファイル
:
JPhysChemB_106_2002_12052.pdf (全文テキスト(全般)), 122KB
 
ファイルのパーマリンク:
-
ファイル名:
JPhysChemB_106_2002_12052.pdf
説明:
-
OA-Status:
閲覧制限:
制限付き (Max Planck Institute for Medical Research, MHMF; )
MIMEタイプ / チェックサム:
application/pdf
技術的なメタデータ:
著作権日付:
-
著作権情報:
-
CCライセンス:
-

関連URL

表示:
非表示:
URL:
http://dx.doi.org/10.1021/jp0265743 (全文テキスト(全般))
説明:
-
OA-Status:
説明:
-
OA-Status:

作成者

表示:
非表示:
 作成者:
Pushkar, Yulia N., 著者
Zech, Stephan G., 著者
Stehlik, Dietmar, 著者
Brown, Sarah, 著者
van der Est, Art, 著者
Zimmermann, Herbert1, 著者           
所属:
1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

内容説明

表示:
非表示:
キーワード: -
 要旨: 1,4-Naphthoquinone (NQ) and its monosubstituted derivatives, 2-methyl, 2-ethyl, and 2-butyl-1,4-naphthoquinone, have been incorporated into the A1 binding site of photosystem I (PS I) after organic solvent extraction of the native phylloquinone. The charge separated state P700+NQ- has been studied by multifrequency transient EPR. The Q-band (35 GHz) spectra of fully deuterated 2-methyl-1,4-NQ-d8 and 2-ethyl-1,4-NQ-d10 show sufficient spectral resolution for the orientation of the quinone g tensor and thus the headgroup of the molecule to be determined. All orientation parameters of the substituted NQs are found to be the same as those established for native phylloquinone in PS I. However, for 2-ethyl-1,4-NQ and 2-butyl-1,4-NQ the X- and Q-band spectra exhibit a well resolved 1:2:1 hyperfine splitting. From the fact that it is absent when the first methylene group of the side chain is selectively deuterated, the splitting is assigned to the hyperfine coupling of the methylene protons. The principal values of the axially symmetric hyperfine coupling tensor are determined to be |Axx| = 12.2 MHz, |Ayy| = 16.8 MHz, |Azz| = 12.2 MHz, and aiso = 13.7 MHz. The large methylene proton hyperfine coupling arises from a high spin density on the ring carbon atom to which the alkyl tail is attached. This in turn suggests that only one of the carbonyl groups of 2-alkyl-1,4-NQ is H-bonded to the protein and that the alkyl tail must be in the ortho position relative to the carbonyl group with the H bond. This implies that the alkyl side chain of the substituted NQs resides in the space normally occupied by the methyl group of phylloquinone and not that of the phytyl tail, which is meta to the H-bonded carbonyl group according to the X-ray structure. In addition, the hyperfine tensor indicates that the first two C−C bonds of the alkyl tail must be coplanar with the aromatic ring. However, the X-ray structure of PS I shows, for the native phylloquinone, that the phytyl tail is bent out of the quinone plane with the second C−C bond.

資料詳細

表示:
非表示:
言語: eng - English
 日付: 2002-06-202002-09-202002-10-242002-10-24
 出版の状態: 出版
 ページ: -
 出版情報: -
 目次: -
 査読: 査読あり
 学位: -

関連イベント

表示:

訴訟

表示:

Project information

表示:

出版物 1

表示:
非表示:
出版物名: The Journal of Physical Chemistry B
  その他 : J. Phys. Chem. B
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: Washington, D.C. : American Chemical Society
ページ: - 巻号: 106 (46) 通巻号: - 開始・終了ページ: 12052 - 12058 識別子(ISBN, ISSN, DOIなど): ISSN: 1520-6106
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000293370_1