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  Modification of choline acetyltransferase by integration of green fluorescent protein does not affect enzyme activity and subcellular distribution

Rathenberg, J., Gärtner, A., Koenen, M., & Witzemann, V. (2002). Modification of choline acetyltransferase by integration of green fluorescent protein does not affect enzyme activity and subcellular distribution. Cell and Tissue Research, 308(1), 1-6. doi:10.1007/s00441-002-0543-x.

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Genre: Journal Article
Alternative Title : Modification of choline acetyltransferase by integration of green fluorescent protein does not affect enzyme activity and subcellular distribution

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CellTissRes_308_2002_1.pdf (Any fulltext), 113KB
 
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 Creators:
Rathenberg, Jan1, Author              
Gärtner, Annette, Author
Koenen, Michael1, 2, Author              
Witzemann, Veit1, 2, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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Free keywords: ChAT; GFP; Cholinergic marker; In vivo visualization; Mouse (C57/B16); Cell culture; Rat (Wistar)
 Abstract: Choline acetyltransferase (ChAT) is widely used as a marker enzyme to identify cholinergic neurons in the central and peripheral nervous system and to study developmental changes. In order to visualize expression of ChAT directly we have generated a ChAT-green fluorescent protein (GFP) fusion construct. Upon transfection of COS-1 cells and cultured rat hippocampal neurons, transgenic enzymatically active ChAT-GFP is expressed and shows intrinsic fluorescence. In COS-1 cells the ChAT-GFP construct revealed a subcellular distribution indistinguishable from wild-type ChAT. In primary neurons the fluorescence was present in the soma and neuritic processes. Hence, this construct will be useful for analyzing the expression and subcellular distribution of ChAT-GFP in cell and tissue culture.

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Language(s): eng - English
 Dates: 2001-11-232002-02-052002-04-042002-04-01
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Cell and Tissue Research
Source Genre: Journal
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Publ. Info: Heidelberg : Springer-Verlag
Pages: - Volume / Issue: 308 (1) Sequence Number: - Start / End Page: 1 - 6 Identifier: ISSN: 0302-766X
CoNE: https://pure.mpg.de/cone/journals/resource/991042749577550