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  NMDA receptor 2 (NR2) C-terminal control of NR open probability regulates synaptic transmission and plasticity at a cerebellar synapse

Rossi, P., Sola, E., Taglietti, V., Borchardt, T., Steigerwald, F., Utvik, J. K., et al. (2002). NMDA receptor 2 (NR2) C-terminal control of NR open probability regulates synaptic transmission and plasticity at a cerebellar synapse. The Journal of Neuroscience: the Official Journal of the Society for Neuroscience, 22(22), 9687-9697. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12427824.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-78A0-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-78A1-E
Genre: Journal Article
Alternative Title : NMDA receptor 2 (NR2) C-terminal control of NR open probability regulates synaptic transmission and plasticity at a cerebellar synapse

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 Creators:
Rossi, Paola, Author
Sola, Elisabetta, Author
Taglietti, Vanni, Author
Borchardt, Thilo1, Author              
Steigerwald, Frank1, Author              
Utvik, Jo Kristian, Author
Ottersen, Ole Petter, Author
Köhr, Georg1, Author              
D´Angelo, Egidio, Author
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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Free keywords: NMDA receptor; LTP; channel open probability; cerebellum; granule cell; gene knock-out
 Abstract: The C-terminal domain of NMDA receptor 2 (NR2) subunits has been proposed to play a critical role in regulating NMDA receptor localization and function in postsynaptic densities. However, the mechanism of this regulation is not completely understood. In this paper we show that C-terminal truncation of NR2A and NR2C subunits in mice (NR2A/C(DeltaC/DeltaC)) impairs synaptic transmission and plasticity at the cerebellar mossy fiber-granule cell relay. Activation of synaptic NMDA receptors could be distinguished from that of extrasynaptic receptors by using the glutamate scavenger glutamate pyruvate transaminase and the open channel blocker MK801. NR2A/C(DeltaC/DeltaC) mice exhibited a specific reduction in synaptic NMDA receptor activation attributable to a severalfold decrease in channel open probability but not channel conductance. Immunodetection revealed normal developmental expression of NR subunit proteins. Quantitative immunogold analyses with an antibody to NR1 indicated that the reduction in receptor activation is not attributed to a reduced number of NR1-containing receptors in postsynaptic densities. Thus, NR2A/NR2C subunits and particularly their C termini regulate synaptic NMDA receptor activation and function by enhancing channel open probability, which is critical for long-term potentiation induction.

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Language(s): eng - English
 Dates: 2002-09-062002-04-152002-09-062002-11-15
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
  Other : J. Neurosci.
Source Genre: Journal
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Publ. Info: Baltimore, MD : The Society
Pages: - Volume / Issue: 22 (22) Sequence Number: - Start / End Page: 9687 - 9697 Identifier: ISSN: 0270-6474
CoNE: https://pure.mpg.de/cone/journals/resource/954925502187_1