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  SMC condensin entraps chromosomal DNA by an ATP hydrolysis dependent loading mechanism in Bacillus subtilis

Wilhelm, L., Bürmann, F., Minnen, A., Shin, H.-C., Toseland, C. P., Oh, B.-H., et al. (2015). SMC condensin entraps chromosomal DNA by an ATP hydrolysis dependent loading mechanism in Bacillus subtilis. eLife, 4: e06659. doi:10.7554/eLife.06659.

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Wilhelm, Larissa1, Author              
Bürmann, Frank1, Author              
Minnen, Anita1, Author              
Shin, Ho-Chul, Author
Toseland, Christopher P.1, Author              
Oh, Byung-Ha, Author
Gruber, Stephan1, Author              
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1Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565151              

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 Abstract: Smc–ScpAB forms elongated, annular structures that promote chromosome segrega- tion, presumably by compacting and resolving sister DNA molecules. The mechanistic basis for its action, however, is only poorly understood. Here, we have established a physical assay to determine whether the binding of condensin to native chromosomes in Bacillus subtilis involves entrapment of DNA by the Smc–ScpAB ring. To do so, we have chemically cross-linked the three ring interfaces in Smc–ScpAB and thereafter isolated intact chromosomes under protein denaturing conditions. Exclusively species of Smc–ScpA, which were previously cross-linked into covalent rings, remained associated with chromosomal DNA. DNA entrapment is abolished by mutations that interfere with the Smc ATPase cycle and strongly reduced when the recruitment factor ParB is deleted, implying that most Smc–ScpAB is loaded onto the chromosome at parS sites near the replication origin. We furthermore report a physical interaction between native Smc–ScpAB and chromosomal DNA fragments

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Language(s): eng - English
 Dates: 2015-05-07
 Publication Status: Published online
 Pages: 18
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.7554/eLife.06659
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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 4 Sequence Number: e06659 Start / End Page: - Identifier: Other: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X