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  Crosstalk of cardiomyocytes and fibroblasts in co-cultures

Rother, J., Richter, C., Turco, L., Knoch, F., Mey, I., Luther, S., et al. (2015). Crosstalk of cardiomyocytes and fibroblasts in co-cultures. Open Biology, 5(6): 150038. doi:10.1098/rsob.150038.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-7B2E-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-D86D-C
Genre: Journal Article

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 Creators:
Rother, J., Author
Richter, Claudia1, Author              
Turco, Laura2, Author              
Knoch, Fabian2, Author              
Mey, I., Author
Luther, Stefan1, Author              
Janshoff, A., Author
Bodenschatz, Eberhard2, Author              
Tarantola, Marco2, Author              
Affiliations:
1Research Group Biomedical Physics, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063288              
2Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063287              

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Free keywords: fibrosis; contractile function; fibroblasts; impedance spectroscopy; electric cell-substrate impedance sensing; cardiomyocytes
 Abstract: Electromechanical function of cardiac muscle depends critically on the crosstalk of myocytes with non-myocytes. Upon cardiac fibrosis, fibroblasts translocate into infarcted necrotic tissue and alter their communication capabilities. In the present in vitro study, we determined a multiple parameter space relevant for fibrotic cardiac tissue development comprising the following essential processes: (i) adhesion to substrates with varying elasticity, (ii) dynamics of contractile function, and (iii) electromechanical connectivity. By combining electric cell-substrate impedance sensing (ECIS) with conventional optical microscopy, we could measure the impact of fibroblast–cardiomyocyte ratio on the aforementioned parameters in a non-invasive fashion. Adhesion to electrodes was quantified via spreading rates derived from impedance changes, period analysis allowed us to measure contraction dynamics and modulations of the barrier resistance served as a measure of connectivity. In summary, we claim that: (i) a preferred window for substrate elasticity around 7 kPa for low fibroblast content exists, which is shifted to stiffer substrates with increasing fibroblast fractions. (ii) Beat frequency decreases nonlinearly with increasing fraction of fibroblasts, while (iii) the intercellular resistance increases with a maximal functional connectivity at 75% fibroblasts. For the first time, cardiac cell–cell junction density-dependent connectivity in co-cultures of cardiomyocytes and fibroblasts was quantified using ECIS.

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Language(s): eng - English
 Dates: 2015-06-17
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1098/rsob.150038
BibTex Citekey: Rother2015
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Title: Open Biology
Source Genre: Journal
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Pages: 10 Volume / Issue: 5 (6) Sequence Number: 150038 Start / End Page: - Identifier: ISSN: 2046-2441