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  Codon-improved Cre recombinase (iCre) expression in the mouse

Shimshek, D. R., Kim, J.-H., Hübner, M. R., Spergel, D. J., Buchholz, F., Casanova, J. E., et al. (2002). Codon-improved Cre recombinase (iCre) expression in the mouse. Genesis, 32(1), 19-26. doi:10.1002/gene.10023.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-7BB1-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-7BB2-E
Genre: Journal Article
Alternative Title : Codon-improved Cre recombinase (iCre) expression in the mouse

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Genesis_32_2002_19.pdf (Any fulltext), 3MB
 
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 Creators:
Shimshek, Derya R.1, Author              
Kim, Jin-Hyun1, Author              
Hübner, Michael R., Author
Spergel, Daniel J.1, Author              
Buchholz, Frank, Author
Casanova, James E., Author
Stewart, Andrew Francis, Author
Seeburg, Peter H.1, Author              
Sprengel, Rolf1, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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Free keywords: iCre recombinase; loxP; GnRH promoter; LBD; ER
 Abstract: By applying the mammalian codon usage to Cre recombinase, we improved Cre expression, as determined by immunoblot and functional analysis, in three different mammalian cell lines. The improved Cre (iCre) gene was also designed to reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals. Transgenic iCre expressing mice were obtained with good frequency, and in these mice loxP-mediated DNA recombination was observed in all cells expressing iCre. Moreover, iCre fused to two estrogen receptor hormone binding domains for temporal control of Cre activity could also be expressed in transgenic mice. However, Cre induction after administration of tamoxifen yielded only low Cre activity. Thus, whereas efficient activation of Cre fusion proteins in the brain needs further improvements, our studies indicate that iCre should facilitate genetic experiments in the mouse.

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Language(s): eng - English
 Dates: 2001-09-242001-11-202002-01-082002-01-01
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Genesis
Source Genre: Journal
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Publ. Info: New York, NY : Wiley-Liss
Pages: - Volume / Issue: 32 (1) Sequence Number: - Start / End Page: 19 - 26 Identifier: ISSN: 1526-954X
CoNE: https://pure.mpg.de/cone/journals/resource/954925489840