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  Mutations in the relay loop region result in dominant-negative inhibition of myosin II function in Dictyostelium

Tsiavaliaris, G., Fujita-Becker, S., Batra, R., Levitsky, D. I., Kull, F. J., Geeves, M. A., et al. (2002). Mutations in the relay loop region result in dominant-negative inhibition of myosin II function in Dictyostelium. EMBO Reports, 3(11), 1099-1105. doi:10.1093/embo-reports/kvf214.

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Genre: Journal Article
Alternative Title : Mutations in the relay loop region result in dominant-negative inhibition of myosin II function in Dictyostelium

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EMBORep_3_2002_1099.pdf (Any fulltext), 488KB
 
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Tsiavaliaris, Georgios1, Author              
Fujita-Becker, Setsuko1, Author              
Batra, Renu1, Author              
Levitsky, Dmitrii I., Author
Kull, F. Jon, Author
Geeves, Michael A., Author
Manstein, Dietmar J.1, Author              
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1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: Dominant-negative inhibition is a powerful genetic tool for the characterization of gene function in vivo, based on the specific impairment of a gene product by the coexpression of a mutant version of the same gene product. We describe the detailed characterization of two myosin constructs containing either point mutations F487A or F506G in the relay region. Dictyostelium cells transformed with F487A or F506G myosin are unable to undergo processes that require myosin II function, including fruiting-body formation, normal cytokinesis and growth in suspension. Our results show that the dominant-negative inhibition of myosin function is caused by disruption of the communication between active site and lever arm, which blocks motor activity completely, and perturbation of the communication between active site and actin-binding site, leading to an ∼100-fold increase in the mutants' affinity for actin in the presence of ATP.

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Language(s): eng - English
 Dates: 2002-08-292002-07-082002-09-042002-11-012002-11
 Publication Status: Published in print
 Pages: 7
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 Rev. Type: Peer
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Title: EMBO Reports
  Other : EMBO Rep.
Source Genre: Journal
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Publ. Info: Oxford, UK : Published for EMBO by Oxford University Press
Pages: - Volume / Issue: 3 (11) Sequence Number: - Start / End Page: 1099 - 1105 Identifier: ISSN: 1469-221X
CoNE: https://pure.mpg.de/cone/journals/resource/110978984569661