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  Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes

Beliveau, B. J., Boettiger, A. N., Avendano, M. S., Jungmann, R., McCole, R. B., Joyce, E. F., et al. (2015). Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes. NATURE COMMUNICATIONS, 6: 7147. doi:10.1038/ncomms8147.

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 Creators:
Beliveau, Brian J.1, Author
Boettiger, Alistair N.1, Author
Avendano, Maier S.1, Author
Jungmann, Ralf2, Author              
McCole, Ruth B.1, Author
Joyce, Eric F.1, Author
Kim-Kiselak, Caroline1, Author
Bantignies, Frederic1, Author
Fonseka, Chamith Y.1, Author
Erceg, Jelena1, Author
Hannan, Mohammed A.1, Author
Hoang, Hien G.1, Author
Colognori, David1, Author
Lee, Jeannie T.1, Author
Shih, William M.1, Author
Yin, Peng1, Author
Zhuang, Xiaowei1, Author
Wu, Chao-ting1, Author
Affiliations:
1external, ou_persistent22              
2Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              

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Free keywords: STRUCTURED-ILLUMINATION MICROSCOPY; ALLELE-SPECIFIC DETECTION; OLIGONUCLEOTIDE PROBES; NUCLEAR ARCHITECTURE; BITHORAX COMPLEX; PADLOCK PROBES; REVEALS; HYBRIDIZATION; RESOLUTION; DYNAMICS
 Abstract: Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances in FISH-based imaging. We first describe the in situ visualization of single-copy regions of the genome using two single-molecule super-resolution methodologies. We then introduce a robust and reliable system that harnesses single-nucleotide polymorphisms (SNPs) to visually distinguish the maternal and paternal homologous chromosomes in mammalian and insect systems. Both of these new technologies are enabled by renewable, bioinformatically designed, oligonucleotide-based Oligopaint probes, which we augment with a strategy that uses secondary oligonucleotides (oligos) to produce and enhance fluorescent signals. These advances should substantially expand the capability to query parent-of-origin-specific chromosome positioning and gene expression on a cell-by-cell basis.

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Language(s): eng - English
 Dates: 2015
 Publication Status: Published online
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000355533700006
DOI: 10.1038/ncomms8147
 Degree: -

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Title: NATURE COMMUNICATIONS
Source Genre: Journal
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Publ. Info: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 6 Sequence Number: 7147 Start / End Page: - Identifier: ISSN: 2041-1723