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  Cytotoxic activity and chemical constituents of Anthemis mirheydari

Jassbi, A. R., Firuzi, O., Miri, R., Salhei, S., Zare, S., Zare, M., et al. (2016). Cytotoxic activity and chemical constituents of Anthemis mirheydari. Pharmaceutical Biology, 54(10), 2044-2049. doi:10.3109/13880209.2016.1141220.

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ITB515.pdf (Publisher version), 776KB
 
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Jassbi, Amir Reza, Author
Firuzi, Omidreza, Author
Miri, Ramin, Author
Salhei, Sajad, Author
Zare, Somaye, Author
Zare, Mehdi, Author
Masroorbabanari, Mehdi, Author
Chandran, Jima N.1, Author              
Schneider, Bernd1, Author              
Baldwin, Ian Thomas2, Author              
Affiliations:
1Research Group Biosynthesis / NMR, MPI for Chemical Ecology, Max Planck Society, ou_421898              
2Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society, ou_24029              

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 Abstract: Context The genus Anthemis L. (Asteraceae) comprises about 195 species which are widely used in the pharmaceutical, cosmetic and food industries. Objective Anthemis mirheydari Iranshar, an endemic plant from Iran, was investigated for its cytotoxic properties and chemical constituents. Materials and methods The whole parts of the plant (320 g) were extracted by dichloromethane and methanol for four days, successively. The cytotoxic activity of both dichloromethane and methanol extracts were assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric methods against three human cancer cell lines including LS180, MCF-7 and MOLT-4. Different concentrations (10–100 μg/mL) of the plant extracts were tested to obtain IC50 values. The dichloromethane extract of A. mirheydari was subjected to silica gel-column and thin layer chromatography for purification of its chemical constituents and the isolated compounds were further tested against MOLT-4 cells. The structures of the pure compounds were elucidated using different spectral data including nuclear magnetic resonance and electron impact mass spectra. Results The IC50 values of the dichloromethane extract were 30.8 ± 6.7, 25.2 ± 6.5 and 8.6 ± 1.1 μg/mL (means ± standard error) for the above-mentioned cell lines, respectively. Two triterpenoids, taraxasterol (1) and pseudotaraxasterol (2), one sterol, β-sitosterol (3) and one coumarin, 7-methoxycoumarin (4) were isolated from the extract. The IC50 of the mixture of compounds 1 and 2 as well as compounds 3 and 4 were higher (>100 μM) than that reported for the dichloromethane extract against MOLT-4 cells.

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 Dates: 2016-01-072016-02-10
 Publication Status: Published online
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 Identifiers: Other: ITB515
DOI: 10.3109/13880209.2016.1141220
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Title: Pharmaceutical Biology
  Abbreviation : Pharm Biol
Source Genre: Journal
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Publ. Info: New York, NY : Informa Healthcare
Pages: - Volume / Issue: 54 (10) Sequence Number: - Start / End Page: 2044 - 2049 Identifier: Other: 1388-0209
CoNE: https://pure.mpg.de/cone/journals/resource/1388-0209