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  Topographic Mapping of the Synaptic Cleft into Adhesive Nanodomains

de Arce, K. P., Schrod, N., Metzbower, S. W. R., Allgeyer, E., Kong, G.-K.-.-W., Tang, A.-H., et al. (2015). Topographic Mapping of the Synaptic Cleft into Adhesive Nanodomains. NEURON, 88(6), 1165-1172. doi:10.1016/j.neuron.2015.11.011.

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 Creators:
de Arce, Karen Perez1, Author
Schrod, Nikolas2, Author           
Metzbower, Sarah W. R.1, Author
Allgeyer, Edward1, Author
Kong, Geoffrey K. - W.2, Author           
Tang, Ai-Hui1, Author
Krupp, Alexander J.1, Author
Stein, Valentin1, Author
Liu, Xinran1, Author
Bewersdorf, Joerg1, Author
Blanpied, Thomas A.1, Author
Lucic, Vladan2, Author           
Biederer, Thomas1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: NEUROLIGIN 1; SYNAPSES; MICROSCOPY; RECEPTORS; COMPLEXES; MOLECULES; JUNCTIONS; DYNAMICS; DENSITY; SYNCAM
 Abstract: The cleft is an integral part of synapses, yet its macromolecular organization remains unclear. We show here that the cleft of excitatory synapses exhibits a distinct density profile as measured by cryoelectron tomography (cryo-ET). Aiming for molecular insights, we analyzed the synapse-organizing proteins Synaptic Cell Adhesion Molecule 1 (SynCAM 1) and EphB2. Cryo-ET of SynCAM 1 knockout and overexpressor synapses showed that this immunoglobulin protein shapes the cleft's edge. SynCAM 1 delineates the postsynaptic perimeter as determined by immunoelectron microscopy and super-resolution imaging. In contrast, the EphB2 receptor tyrosine kinase is enriched deeper within the postsynaptic area. Unexpectedly, SynCAM 1 can form ensembles proximal to postsynaptic densities, and synapses containing these ensembles were larger. Postsynaptic SynCAM 1 surface puncta were not static but became enlarged after a long-term depression paradigm. These results support that the synaptic cleft is organized on a nanoscale into sub-compartments marked by distinct trans-synaptic complexes.

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Language(s): eng - English
 Dates: 2015
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: NEURON
Source Genre: Journal
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Publ. Info: 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 88 (6) Sequence Number: - Start / End Page: 1165 - 1172 Identifier: ISSN: 0896-6273