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  Time-resolved and in-situ X-ray scattering methods beyond photoactivation: Utilizing high-flux X-ray sources for the study of ubiquitous non-photoactive proteins.

Jain, R., & Techert, S. (2016). Time-resolved and in-situ X-ray scattering methods beyond photoactivation: Utilizing high-flux X-ray sources for the study of ubiquitous non-photoactive proteins. Protein and Peptide Letters, 23(3), 242-254. doi:10.2174/0929866523666160106153847.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-AF47-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-CA49-1
Genre: Journal Article

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 Creators:
Jain, R.1, Author              
Techert, S.1, Author              
Affiliations:
1Research Group of Structural Dynamics of (Bio)Chemical Systems, MPI for Biophysical Chemistry, Max Planck Society, ou_578564              

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Free keywords: Biocatalysis, Free electron laser, Guanylate kinase, Microfluidics, Protein dynamics, Protein folding-unfolding, Small-angle X-ray scattering (SAXS), Structural Dynamics, Time-resolved X-ray scattering (TR-SAXS/TR-WAXS), Wideangle X-ray scattering (WAXS), X-rays
 Abstract: X-ray scattering technique, comprising of small-angle/wide-angle X-ray scattering (SAXS/WAXS) techniques is increasingly used to characterize the structure and interactions of biological macromolecules and their complexes in solution. It is a method of choice to characterize the flexible, partially folded and unfolded protein systems. X-ray scattering is the last resort for proteins that cannot be investigated by crystallography or NMR and acts as a complementary technique with different biophysical techniques to answer challenging scientific questions. The marriage of the X-ray scattering technique with the fourth dimension "time" yields structural dynamics and kinetics information for protein motions in hierarchical timescales from picoseconds to days. The arrival of the high-flux X-ray beam at third generation synchrotron sources, exceptional X-ray optics, state-of-the-art detectors, upgradation of X-ray scattering beamlines with microfluidics devices and advanced X-ray scattering data analysis procedures are the important reasons behind the shining years of X-ray scattering technique. The best days of the X-ray scattering technique are on the horizon with the advent of the nanofocus X-ray scattering beamlines and fourth generation X-ray lightsources, i.e., free electron lasers (XFELs). Complementary to the photon-triggered time-resolved X-ray scattering techniques, we will present an overview of the time-resolved and in-situ X-ray scattering techniques for structural dynamics of ubiquitous non-photoactive proteins.

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Language(s): eng - English
 Dates: 2016-01-062016
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.2174/0929866523666160106153847
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Title: Protein and Peptide Letters
Source Genre: Journal
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Pages: - Volume / Issue: 23 (3) Sequence Number: - Start / End Page: 242 - 254 Identifier: -