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  Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes

Thomas, F. A., Visco, I., Petrasek, Z., Heinemann, F., & Schwille, P. (2015). Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes. Data in Brief, 5, 537-541. doi:10.1016/j.dib.2015.10.002.

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Genre: Journal Article
Subtitle : Data Article

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1-s2.0-S2352340915002504-main.pdf (Publisher version), 306KB
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 Creators:
Thomas, Franziska A.1, Author           
Visco, Ilaria1, Author           
Petrasek, Zdenek1, Author           
Heinemann, Fabian1, Author           
Schwille, Petra1, Author           
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1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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 Abstract: Recently, a new and versatile assay to determine the partitioning coef fi cient K P as a measure for the af fi nity of peripheral membrane proteins for lipid bilayers was presented in the research article enti- tled, “ Introducing a fl uorescence-based standard to quantify protein partitioning into membranes ” [1] . Here, the well-characterized bind- ing of hexahistidine-tag (His 6 ) to NTA(Ni) was utilized. Com- plementarily, this data article reports the average diffusion coef fi cient D of His 6 -tagged enhanced green fl uorescent protein (eGFP-His 6 )and the fl uorescent lipid analog ATTO ‐ 647N ‐ DOPE in giant unilamellar vesicles (GUVs) containing different amounts of NTA(Ni) lipids. In addition, dissociation constants K d of the NTA(Ni)/eGFP-His 6 system are reported. Further, a conversion between K d and K P is provided

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Language(s): eng - English
 Dates: 2015
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: Refers to
Franziska A. Thomas, Ilaria Visco, Zdeněk Petrášek, Fabian Heinemann, Petra Schwille
Introducing a fluorescence-based standard to quantify protein partitioning into membranes
Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1848, Issue 11, Part A, November 2015, Pages 2932-2941
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 Identifiers: DOI: 10.1016/j.dib.2015.10.002
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Title: Data in Brief
  Abbreviation : Data Brief
Source Genre: Journal
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Publ. Info: Dordrecht : Elsevier
Pages: - Volume / Issue: 5 Sequence Number: - Start / End Page: 537 - 541 Identifier: ISSN: 2352-3409
CoNE: https://pure.mpg.de/cone/journals/resource/2352-3409