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  Regulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement

Budkevich, T. V., Giesebrecht, J., Behrmann, E., Loerke, J., Ramrath, D. J., Mielke, T., et al. (2014). Regulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement. Cell, 158(1), 121-131. doi:10.1016/j.cell.2014.04.044.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-B3E3-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-B3E4-D
Genre: Journal Article

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 Creators:
Budkevich, T. V.1, 2, 3, Author
Giesebrecht, J.1, Author
Behrmann, E.1, Author
Loerke, J.1, Author
Ramrath, D. J.1, Author
Mielke, T.1, 4, Author              
Ismer, J.1, Author
Hildebrand, P. W.1, Author
Tung, C. S.5, Author
Nierhaus, K. H.1, 2, Author
Sanbonmatsu, K. Y.5, 6, Author
Spahn, C. M.1, Author
Affiliations:
1Institut für Medizinische Physik und Biophysik, Charité–Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany, ou_persistent22              
2Max-Planck Institut für Molekulare Genetik, Abteilung Vingron, AG Ribosomen, 14195 Berlin, Ihnestraße 73, Germany, ou_persistent22              
3Institute of Molecular Biology and Genetics, Group of Protein Biosynthesis, 03143 Kiev, Ukraine, ou_persistent22              
4Microscopy and Cryo-Electron Microscopy (Head: Thorsten Mielke), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              
5Theoretical Biology and Biophysics Group, Theoretical Division, Los Alamos National Laboratory, MK710, Los Alamos, NM 87545, USA, ou_persistent22              
6New Mexico Consortium, 4200 West Jemez Road, Suite 301, Los Alamos, New Mexico 87544, USA, ou_persistent22              

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Free keywords: Amino Acid Sequence Animals Anticodon/metabolism Codon/metabolism Cryoelectron Microscopy Crystallography, X-Ray Humans Mammals/*metabolism Molecular Sequence Data Peptide Chain Elongation, Translational RNA, Transfer/metabolism Rabbits Ribosomes/*chemistry Saccharomyces cerevisiae/metabolism Tetrahymena thermophila/metabolism
 Abstract: The extent to which bacterial ribosomes and the significantly larger eukaryotic ribosomes share the same mechanisms of ribosomal elongation is unknown. Here, we present subnanometer resolution cryoelectron microscopy maps of the mammalian 80S ribosome in the posttranslocational state and in complex with the eukaryotic eEF1AVal-tRNAGMPPNP ternary complex, revealing significant differences in the elongation mechanism between bacteria and mammals. Surprisingly, and in contrast to bacterial ribosomes, a rotation of the small subunit around its long axis and orthogonal to the well-known intersubunit rotation distinguishes the posttranslocational state from the classical pretranslocational state ribosome. We term this motion "subunit rolling." Correspondingly, a mammalian decoding complex visualized in substates before and after codon recognition reveals structural distinctions from the bacterial system. These findings suggest how codon recognition leads to GTPase activation in the mammalian system and demonstrate that in mammalia subunit rolling occurs during tRNA selection.

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Language(s): eng - English
 Dates: 2014-07-03
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.cell.2014.04.044
ISSN: 1097-4172 (Electronic)
ISSN: 0092-8674 (Print)
 Degree: -

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Title: Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 158 (1) Sequence Number: - Start / End Page: 121 - 131 Identifier: ISSN: 0092-8674
CoNE: https://pure.mpg.de/cone/journals/resource/954925463183