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  Assembly of Helicobacter pylori initiation complex is determined by sequence-specific and topology-sensitive DnaA-oriC interactions

Donczew, R., Mielke, T., Jaworski, P., Zakrzewska-Czerwińska, J., & Zawilak-Pawlik, A. (2014). Assembly of Helicobacter pylori initiation complex is determined by sequence-specific and topology-sensitive DnaA-oriC interactions. Journal of Molecular Biology (London), 426(15), 2769-2782. doi:10.1016/j.jmb.2014.05.018.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-BBE8-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-BBE9-0
Genre: Journal Article

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 Creators:
Donczew, Rafał 1, Author
Mielke, Thorsten2, Author              
Jaworski, Paweł 1, Author
Zakrzewska-Czerwińska, Jolanta1, 3, Author
Zawilak-Pawlik, Anna 1, Author
Affiliations:
1Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Department of Microbiology, Weigla 12, 53-114 Wrocław, Poland, ou_persistent22              
2Microscopy and Cryo-Electron Microscopy (Head: Thorsten Mielke), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              
3University of Wrocław, Faculty of Biotechnology, Joliot-Curie 14a, 50-138 Wrocław, Poland, ou_persistent22              

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Free keywords: DNA replication; orisome formation; DNA topology; DNA sequence; oriC
 Abstract: In bacteria, chromosome replication is initiated by binding of the DnaA initiator protein to DnaA boxes located in the origin of chromosomal replication (oriC). This leads to DNA helix opening within the DNA-unwinding element. Helicobacter pylori oriC, the first bipartite origin identified in Gram-negative bacteria, contains two subregions, oriC1 and oriC2, flanking the dnaA gene. The DNA-unwinding element region is localized in the oriC2 subregion downstream of dnaA. Surprisingly, oriC2–DnaA interactions were shown to depend on DNA topology, which is unusual in bacteria but is similar to initiator–origin interactions observed in higher organisms. In this work, we identified three DnaA boxes in the oriC2 subregion, two of which were bound only as supercoiled DNA. We found that all three DnaA boxes play important roles in orisome assembly and subsequent DNA unwinding, but different functions can be assigned to individual boxes. This suggests that the H. pylori oriC may be functionally divided, similar to what was described recently for Escherichia coli oriC. On the basis of these results, we propose a model of initiation complex formation in H. pylori.

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Language(s): eng - English
 Dates: 2014-05-162014-05-242014-07-29
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.jmb.2014.05.018
 Degree: -

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Title: Journal of Molecular Biology (London)
  Other : J Mol Biol
Source Genre: Journal
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Publ. Info: London : Academic Press
Pages: - Volume / Issue: 426 (15) Sequence Number: - Start / End Page: 2769 - 2782 Identifier: ISSN: 0022-2836
CoNE: https://pure.mpg.de/cone/journals/resource/954922646042