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  Atxn2-Knock-Out mice show branched chain amino acids and fatty acids pathway alterations

Meierhofer, D., Halbach, M., Şen, N. E., Gispert, S., & Auburger, G. (2016). Atxn2-Knock-Out mice show branched chain amino acids and fatty acids pathway alterations. Molecular and Cellular Proteomics, 15(5), 1728-1739. doi:10.1074/mcp.M115.056770.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-BE06-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-135A-D
Genre: Journal Article

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 Creators:
Meierhofer, David1, Author              
Halbach, Melanie2, Author
Şen, Nesli Ece 2, Author
Gispert, Suzana 2, Author
Auburger, Georg 2, Author
Affiliations:
1Mass Spectrometry (Head: David Meierhofer), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479669              
2 Exp. Neurology, Building 89, Goethe University Medical School, Theodor Stern Kai 7, 60590 Frankfurt am Main, Germany , ou_persistent22              

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Free keywords: Spinocerebellar Ataxia type 2 (Atxn2); mass spectrometry; proteome profiling; metabolome profiling; multiple reaction monitoring (MRM); protein - protein interaction network (PPI) analysis; pathway analysis
 Abstract: Human Ataxin-2 (ATXN2) gene locus variants have been associated with obesity, diabetes mellitus type 1 and hypertension in genome-wide association studies, while mouse studies showed the knock-out of Atxn2 to lead to obesity, insulin resistance and dyslipidemia. Intriguingly, the deficiency of ATXN2 protein orthologues in yeast and flies rescues the neurodegeneration process triggered by TDP-43 and Ataxin-1 toxicity. To understand the molecular effects of ATXN2 deficiency by unbiased approaches, we quantified the global proteome and metabolome of Atxn2-knock-out mice with label-free mass spectrometry. In liver tissue, significant downregulations of the proteins ACADS, ALDH6A1, ALDH7A1, IVD, MCCC2, PCCA, OTC, together with bioinformatic enrichment of downregulated pathways for branched chain and other amino acid metabolism, fatty acids and citric acid cycle were observed. Statistical trends in the cerebellar proteome and in the metabolomic profiles supported these findings. They are in good agreement with recent claims that PBP1, the yeast orthologue of ATXN2, sequestrates the nutrient sensor TORC1 in periods of cell stress. Overall, ATXN2 appears to modulate nutrition and metabolism, and its activity changes are determinants of growth excess or cell atrophy.

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Language(s): eng - English
 Dates: 2016-02-052016-05
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: PII: mcp.M115.056770
PMID: 26850065
DOI: 10.1074/mcp.M115.056770
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Title: Molecular and Cellular Proteomics
Source Genre: Journal
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Publ. Info: Bethesda, MD : American Society for Biochemistry and Molecular Biology
Pages: - Volume / Issue: 15 (5) Sequence Number: - Start / End Page: 1728 - 1739 Identifier: ISSN: 1535-9476
CoNE: /journals/resource/111035577487002