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  Synaptically activated Ca2+ waves in layer 2/3 and layer 5 rat neocortical pyramidal neurons

Larkum, M. E., Watanabe, S., Nakamura, T., Lasser-Ross, N., & Nechama, W. N. (2003). Synaptically activated Ca2+ waves in layer 2/3 and layer 5 rat neocortical pyramidal neurons. The Journal of Physiology - London, 549(2), 471-488. doi:10.1113/jphysiol.2002.037614.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-BF6B-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-BF72-D
Genre: Journal Article
Alternative Title : Synaptically activated Ca2+ waves in layer 2/3 and layer 5 rat neocortical pyramidal neurons

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 Creators:
Larkum, Matthew E.1, Author              
Watanabe, Shigeo, Author
Nakamura, Takeshi, Author
Lasser-Ross, Nechama, Author
Nechama, William N., Author
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: Calcium waves in layer 2/3 and layer 5 neocortical somatosensory pyramidal neurons were examined in slices from 2- to 8-week-old rats. Repetitive synaptic stimulation evoked a delayed, all-or-none [Ca2+]i increase primarily on the main dendritic shaft. This component was blocked by 1 mM (R,S)-alpha-methyl-4-carboxyphenylglycine (MCPG), 10 microM ryanodine, 1 mg ml-1 internal heparin, and was not blocked by 400 microM internal Ruthenium Red, indicating that it was due to Ca2+ release from internal stores by inositol 1,4,5-trisphosphate (IP3) mobilized via activation of metabotropic glutamate receptors. Calcium waves were initiated on the apical shaft at sites between the soma to around the main branch point, mostly at insertion points of oblique dendrites, and spread in both directions along the shaft. In the proximal dendrites the peak amplitude of the resulting [Ca2+]i change was much larger than that evoked by a train of Na+ spikes. In distal dendrites the peak amplitude was comparable to the [Ca2+]i change due to a Ca2+ spike. IP3-mediated Ca2+ release also was observed in the presence of the metabotropic agonists t-ACPD and carbachol when backpropagating spikes were generated. Ca2+ entry through NMDA receptors was observed primarily on the oblique dendrites. The main differences between waves in neocortical neurons and in previously described hippocampal pyramidal neurons were, (a) Ca2+ waves in L5 neurons could be evoked further out along the main shaft, (b) Ca2+ waves extended slightly further out into the oblique dendrites and (c) higher concentrations of bath-applied t-ACPD and carbachol were required to generate Ca2+ release events by backpropagating action potentials.

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Language(s): eng - English
 Dates: 2002-12-132003-03-182003-04-112003-06-01
 Publication Status: Published in print
 Pages: 18
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 Rev. Method: Peer
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Title: The Journal of Physiology - London
Source Genre: Journal
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Publ. Info: London : Cambridge University Press
Pages: - Volume / Issue: 549 (2) Sequence Number: - Start / End Page: 471 - 488 Identifier: ISSN: 0022-3751
CoNE: https://pure.mpg.de/cone/journals/resource/954925334693_2