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  Targeted whole-cell recordings in the mammalian brain in vivo

Margrie, T. W., Meyer, A. H., Caputi, A., Monyer, H., Hasan, M. T., Schaefer, A. T., et al. (2003). Targeted whole-cell recordings in the mammalian brain in vivo. Neuron, 39(6), 911-918. doi:10.1016/j.neuron.2003.08.012.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-C66E-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-C66F-D
Genre: Journal Article
Alternative Title : Targeted whole-cell recordings in the mammalian brain in vivo

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 Creators:
Margrie, Troy W.1, 2, Author              
Meyer, Axel H., Author
Caputi, Antonio2, Author              
Monyer, Hannah2, Author              
Hasan, Mazahir T.2, 3, Author              
Schaefer, Andreas T.1, Author              
Denk, Winfried3, Author              
Brecht, Michael1, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
3Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: While electrophysiological recordings from visually identified cell bodies or dendrites are routinely performed in cell culture and acute brain slice preparations, targeted recordings from the mammalian nervous system are currently not possible in vivo. The "blind" approach that is used instead is somewhat random and largely limited to common neuronal cell types. This approach prohibits recordings from, for example, molecularly defined and/or disrupted populations of neurons. Here we describe a method, which we call TPTP (two-photon targeted patching), that uses two-photon imaging to guide in vivo whole-cell recordings to individual, genetically labeled cortical neurons. We apply this technique to obtain recordings from genetically manipulated, parvalbumin-EGFP-positive interneurons in the somatosensory cortex. We find that both spontaneous and sensory-evoked activity patterns involve the synchronized discharge of electrically coupled interneurons. TPTP applied in vivo will therefore provide new insights into the molecular control of neuronal function at the systems level.

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Language(s): eng - English
 Dates: 2003-01-212003-08-122003-09-11
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
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Title: Neuron
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 39 (6) Sequence Number: - Start / End Page: 911 - 918 Identifier: ISSN: 0896-6273
CoNE: https://pure.mpg.de/cone/journals/resource/954925560565