English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Molecular architecture of the human U4/U6.U5 tri-snRNP.

Agafonov, D. E., Kastner, B., Dybkov, O., Hofele, R. V., Liu, W., Urlaub, H., et al. (2016). Molecular architecture of the human U4/U6.U5 tri-snRNP. Science, 351(6280), 1416-1420. doi:10.1126/science.aad2085.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-C7F5-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-9182-0
Genre: Journal Article

Files

show Files
hide Files
:
2253195.pdf (Publisher version), 3MB
 
File Permalink:
-
Name:
2253195.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2253195_Suppl.htm (Supplementary material), 51KB
Name:
2253195_Suppl.htm
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/xhtml+xml / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2253195_Suppl_1.pdf (Supplementary material), 6MB
Name:
2253195_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2253195_Suppl_2.xlsx (Supplementary material), 69KB
Name:
2253195_Suppl_2.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2253195_Suppl_3.mp4 (Supplementary material), 30MB
Name:
2253195_Suppl_3.mp4
Description:
-
Visibility:
Public
MIME-Type / Checksum:
video/mp4 / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Agafonov, D. E.1, Author              
Kastner, B.1, Author              
Dybkov, O.1, Author              
Hofele, R. V.2, Author              
Liu, W.3, Author              
Urlaub, H.2, Author              
Lührmann, R.1, Author              
Stark, H.3, Author              
Affiliations:
1Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578576              
2Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
3Department of Structural Dynamics, MPI for Biophysical Chemistry, Max Planck Society, ou_2205645              

Content

show
hide
Free keywords: -
 Abstract: The U4/U6.U5 triple small nuclear ribonucleoprotein (tri-snRNP) is a major spliceosome building block. Here we describe a 7 Å 3D structure of the 1.8 MDa human tri-snRNP obtained by single-particle electron cryomicroscopy. We fit all known high-resolution structures of tri-snRNP components into the EM density map and validated them by protein crosslinking. Our model reveals how the spatial organization of Brr2 RNA helicase prevents pre-mature U4/U6 RNA unwinding in isolated human tri-snRNPs and how the Sad1 protein likely tethers Brr2 to its pre-activation position. Comparison of our model with cryo-EM 3D structures of the S. cerevisiae tri-snRNP and S. pombe spliceosome indicates that Brr2 undergoes a dramatic conformational change during spliceosome activation, and that Prp8 is also rearranged to accommodate the spliceosome's catalytic RNA network.

Details

show
hide
Language(s): eng - English
 Dates: 2016-02-182016-03-25
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1126/science.aad2085
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Science
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 351 (6280) Sequence Number: - Start / End Page: 1416 - 1420 Identifier: -