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  A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization.

van der Velde, J. H. M., Oelerich, J., Huang, J. Y., Smit, J. H., Jazi, A. A., Galiani, S., et al. (2016). A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization. Nature Communications, 7: 10144. doi:10.1038/ncomms10144.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-D497-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-2689-5
Genre: Journal Article

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 Creators:
van der Velde, J. H. M., Author
Oelerich, J., Author
Huang, J. Y., Author
Smit, J. H., Author
Jazi, A. A., Author
Galiani, S., Author
Kolmakov, K.1, Author              
Guoridis, G., Author
Eggeling, C.1, Author              
Herrmann, A., Author
Roelfes, G., Author
Cordes, T., Author
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Intramolecular photostabilization via triple-state quenching was recently revived as a tool to impart synthetic organic fluorophores with ‘self-healing’ properties. To date, utilization of such fluorophore derivatives is rare due to their elaborate multi-step synthesis. Here we present a general strategy to covalently link a synthetic organic fluorophore simultaneously to a photostabilizer and biomolecular target via unnatural amino acids. The modular approach uses commercially available starting materials and simple chemical transformations. The resulting photostabilizer–dye conjugates are based on rhodamines, carbopyronines and cyanines with excellent photophysical properties, that is, high photostability and minimal signal fluctuations. Their versatile use is demonstrated by single-step labelling of DNA, antibodies and proteins, as well as applications in single-molecule and super-resolution fluorescence microscopy. We are convinced that the presented scaffolding strategy and the improved characteristics of the conjugates in applications will trigger the broader use of intramolecular photostabilization and help to emerge this approach as a new gold standard.

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Language(s): eng - English
 Dates: 2016-01-11
 Publication Status: Published online
 Pages: -
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/ncomms10144
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Title: Nature Communications
Source Genre: Journal
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Pages: 15 Volume / Issue: 7 Sequence Number: 10144 Start / End Page: - Identifier: -