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  A mode of action of glucosinolate-derived isothiocyanates: Detoxification depletes glutathione and cysteine levels with ramifications on protein metabolism in Spodoptera littoralis

Jeschke, V., Gershenzon, J., & Giddings Vassão, D. (2016). A mode of action of glucosinolate-derived isothiocyanates: Detoxification depletes glutathione and cysteine levels with ramifications on protein metabolism in Spodoptera littoralis. Insect Biochemistry and Molecular Biology, 71, 37-48. doi:10.1016/j.ibmb.2016.02.002.

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Jeschke, Verena1, 2, Author              
Gershenzon, Jonathan1, Author              
Giddings Vassão, Daniel1, Author              
Affiliations:
1Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, ou_421893              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

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 Abstract: Glucosinolates are activated plant defenses common in the order Brassicales that release isothiocyanates (ITCs) and other hydrolysis products upon tissue damage. The reactive ITCs are toxic to insects resulting in reduced growth, delayed development and occasionally mortality. Generalist lepidopteran larvae often detoxify ingested ITCs via conjugation to glutathione (GSH) and survive on low glucosinolate diets, but it is not known how this process influences other aspects of metabolism. We investigated the impact of the aliphatic 4-methylsulfinylbutyl-ITC (4msob-ITC, sulforaphane) on the metabolism of Spodoptera littoralis larvae, which suffer a significant growth decline on 4msob-ITC-containing diets while excreting ITC-glutathione conjugates and their derivatives in the frass. The most striking effects were a decrease of GSH in midgut tissue and hemolymph due to losses by conjugation to ITC during detoxification, and a decline of the GSH biosynthetic precursor cysteine. Protein content was likewise reduced by ITC treatment suggesting that protein is actively catabolized in an attempt to supply cysteine for GSH biosynthesis. The negative growth and protein effects were relieved by dietary supplementation with cystine. Other consequences of protein breakdown included deamination of amino acids with increased excretion of uric acid and elevated lipid content. Thus metabolic detoxification of ITCs provokes a cascade of negative effects on insects that result in reduced fitness.

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 Dates: 2015-11-182016-02-032016-02-192016
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: Other: GER438
DOI: 10.1016/j.ibmb.2016.02.002
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Title: Insect Biochemistry and Molecular Biology
  Other : Insect Biochem. Mol. Biol.
Source Genre: Journal
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Publ. Info: Oxford [England] : Pergamon
Pages: 12 Volume / Issue: 71 Sequence Number: - Start / End Page: 37 - 48 Identifier: ISSN: 0965-1748
CoNE: https://pure.mpg.de/cone/journals/resource/954925581163