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  Manipulation of interrenal cell function in developing zebrafish using genetically zargeted ablation and an optogenetic tool

Gutierrez-Triana, J. A., Herget, U., Castillo Ramirez, L. A., Lutz, M., Yeh, C.-M., De Marco, R., et al. (2015). Manipulation of interrenal cell function in developing zebrafish using genetically zargeted ablation and an optogenetic tool. Endocrinology, 156(9), 3394-3401. doi:10.1210/EN.2015-1021.

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 Urheber:
Gutierrez-Triana, José Arturo1, Autor           
Herget, Ulrich1, Autor           
Castillo Ramirez, Luis Alberto1, Autor           
Lutz, Markus1, Autor           
Yeh, Chen-Min1, Autor           
De Marco, Rodrigo1, Autor           
Ryu, Soojin1, Autor           
Affiliations:
1Max Planck Research Group Developmental Genetics of the nervous system (Soojin Ryu), Max Planck Institute for Medical Research, Max Planck Society, ou_1497724              

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 Zusammenfassung: Zebrafish offer an opportunity to study conserved mechanisms underlying the ontogeny and physiology of the hypothalamic-pituitary-adrenal/interrenal axis. As the final effector of the hypothalamic-pituitary-adrenal/interrenal axis, glucocorticoids exert both rapid and long-term regulatory functions. To elucidate their specific effects in zebrafish, transgenic approaches are necessary to complement pharmacological studies. Here, we report a robust approach to specifically manipulate endogenous concentrations of cortisol by targeting heterologous proteins to interrenal cells using a promoter element of the steroidogenic acute regulatory protein. To test this approach, we first used this regulatory region to generate a transgenic line expressing the bacterial nitroreductase protein, which allows conditional targeted ablation of interrenal cells. We demonstrate that this line can be used to specifically ablate interrenal cells, drastically reducing both basal and stress-induced cortisol concentrations. Next, we coupled this regulatory region to an optogenetic actuator, Beggiatoa photoactivated adenylyl cyclase, to increase endogenous cortisol concentrations in a blue light-dependent manner. Thus, our approach allows specific manipulations of steroidogenic interrenal cell activity for studying the effects of both hypo- and hypercortisolemia in zebrafish.

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Sprache(n): eng - English
 Datum: 2015-01-112015-06-252015-07-012015-09-01
 Publikationsstatus: Erschienen
 Seiten: 8
 Ort, Verlag, Ausgabe: -
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 Art der Begutachtung: Expertenbegutachtung
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Titel: Endocrinology
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Los Angeles, Calif. : Association for the Study of Internal Secretions
Seiten: - Band / Heft: 156 (9) Artikelnummer: - Start- / Endseite: 3394 - 3401 Identifikator: ISSN: 0013-7227
CoNE: https://pure.mpg.de/cone/journals/resource/991042745737620