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  Fluorescent rhodamines and fluorogenic carbopyronines for super-resolution STED microscopy in living cells.

Butkevich, A., Mitronova, G., Sidenstein, S., Klocke, J., Kamin, D., Meineke, D. N. H., et al. (2016). Fluorescent rhodamines and fluorogenic carbopyronines for super-resolution STED microscopy in living cells. Angewandte Chemie International Edition, 55(10), 3290-3294. doi:10.1002/anie.201511018.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-041D-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-9C65-D
Genre: Journal Article

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Butkevich, A.1, Author              
Mitronova, G.1, Author              
Sidenstein, S.1, Author              
Klocke, J.1, Author              
Kamin, D.1, Author              
Meineke, D. N. H.1, Author              
D'Este, E.1, Author              
Kraemer, P. T.1, Author              
Danzl, J. G.1, Author              
Belov, V. N.1, Author              
Hell, S. W.1, Author              
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1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60 nm.

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Language(s): eng - English
 Dates: 2016-02-042016-03-01
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1002/anie.201511018
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Title: Angewandte Chemie International Edition
Source Genre: Journal
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Pages: - Volume / Issue: 55 (10) Sequence Number: - Start / End Page: 3290 - 3294 Identifier: -