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  Single-vesicle imaging reveals different transport mechanisms between glutamatergic and GABAergic vesicles.

Farsi, Z., Preobraschenski, J., van den Bogaart, G., Riedel, D., Jahn, R., & Woehler, A. (2016). Single-vesicle imaging reveals different transport mechanisms between glutamatergic and GABAergic vesicles. Science, 351(6276), 981-984. doi:10.1126/science.aad8142.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-04EA-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-A78C-F
Genre: Journal Article

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 Creators:
Farsi, Z.1, Author              
Preobraschenski, J.1, Author              
van den Bogaart, G.1, Author              
Riedel, D.2, Author              
Jahn, R.1, Author              
Woehler, A.3, Author              
Affiliations:
1Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              
2Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              
3Emeritus Group of Membrane Biophysics, MPI for Biophysical Chemistry, Max Planck Society, ou_1571137              

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 Abstract: Synaptic transmission is mediated by the release of neurotransmitters, which involves exo-endocytotic cycling of synaptic vesicles. To maintain synaptic function, synaptic vesicles are refilled with thousands of neurotransmitter molecules within seconds after endocytosis, using the energy provided by an electrochemical proton gradient. However, it is unclear how transmitter molecules carrying different net charges can be efficiently sequestered while maintaining charge neutrality and osmotic balance. We used single-vesicle imaging to monitor pH and electrical gradients and directly showed different uptake mechanisms for glutamate and g-aminobutyric acid (GABA) operating in parallel. In contrast to glutamate, GABA was exchanged for protons, with no other ions participating in the transport cycle. Thus, only a few components are needed to guarantee reliable vesicle filling with different neurotransmitters.

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Language(s): eng - English
 Dates: 2016-02-262016-02
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1126/science.aad8142
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Title: Science
Source Genre: Journal
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Pages: - Volume / Issue: 351 (6276) Sequence Number: - Start / End Page: 981 - 984 Identifier: -