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  High-Affinity Sites Form an Interaction Network to Facilitate Spreading of the MSL Complex across the X Chromosome in Drosophila

Ramirez, F., Lingg, T., Toscano, S., Lam, K. C., Georgiev, P., Chung, H. R., et al. (2015). High-Affinity Sites Form an Interaction Network to Facilitate Spreading of the MSL Complex across the X Chromosome in Drosophila. Molecular Cell, 60(1), 146-162. doi:10.1016/j.molcel.2015.08.024.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-58E4-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-58E5-3
Genre: Journal Article

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 Creators:
Ramirez, F., Author
Lingg, T., Author
Toscano, S., Author
Lam, K. C., Author
Georgiev, P., Author
Chung, H. R.1, Author              
Lajoie, B. R., Author
de Wit, E., Author
Zhan, Y., Author
de Laat, W., Author
Dekker, J., Author
Manke, T., Author
Akhtar, A., Author
Affiliations:
1Computational Epigenetics (Ho-Ryun Chung), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479658              

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Free keywords: Animals Binding Sites Cell Line Chromatin Assembly and Disassembly Cytogenetic Analysis DNA-Binding Proteins/*metabolism Dosage Compensation, Genetic Drosophila Proteins/genetics/*metabolism Drosophila melanogaster/*genetics/metabolism Female Male RNA-Binding Proteins/genetics/*metabolism Transcription Factors/genetics/*metabolism X Chromosome/genetics/*metabolism
 Abstract: Dosage compensation mechanisms provide a paradigm to study the contribution of chromosomal conformation toward targeting and spreading of epigenetic regulators over a specific chromosome. By using Hi-C and 4C analyses, we show that high-affinity sites (HAS), landing platforms of the male-specific lethal (MSL) complex, are enriched around topologically associating domain (TAD) boundaries on the X chromosome and harbor more long-range contacts in a sex-independent manner. Ectopically expressed roX1 and roX2 RNAs target HAS on the X chromosome in trans and, via spatial proximity, induce spreading of the MSL complex in cis, leading to increased expression of neighboring autosomal genes. We show that the MSL complex regulates nucleosome positioning at HAS, therefore acting locally rather than influencing the overall chromosomal architecture. We propose that the sex-independent, three-dimensional conformation of the X chromosome poises it for exploitation by the MSL complex, thereby facilitating spreading in males.

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Language(s): eng - English
 Dates: 2015-10-01
 Publication Status: Published in print
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1016/j.molcel.2015.08.024
ISSN: 1097-4164 (Electronic)1097-2765 (Print)
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Title: Molecular Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 60 (1) Sequence Number: - Start / End Page: 146 - 162 Identifier: ISSN: 1097-2765
CoNE: /journals/resource/954925610929