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  Quantitative assessment of ribosome drop-off in E. coli

Sin, C., Chiarugi, D., & Valleriani, A. (2016). Quantitative assessment of ribosome drop-off in E. coli. Nucleic Acids Research (London), 44(6), 2528-2537. doi:10.1093/nar/gkw137.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-10B9-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-3832-8
Genre: Journal Article

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 Creators:
Sin, Celine1, Author              
Chiarugi, Davide1, Author              
Valleriani, Angelo1, Author              
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1Angelo Valleriani, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863324              

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Free keywords: Open Access
 Abstract: Premature ribosome drop-off is one of the major errors in translation of mRNA by ribosomes. However, repeated analyses of Ribo-seq data failed to quantify its strength in E. coli. Relying on a novel highly sensitive data analysis method we show that a significant rate of ribosome drop-off is measurable and can be quantified also when cells are cultured under non-stressing conditions. Moreover, we find that the drop-off rate is highly variable, depending on multiple factors. In particular, under environmental stress such as amino acid starvation or ethanol intoxication, the drop-off rate markedly increases.

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 Dates: 2016-03-012016-04-07
 Publication Status: Published in print
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Title: Nucleic Acids Research (London)
  Other : Nucleic Acids Res
Source Genre: Journal
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Publ. Info: Oxford : Oxford University Press
Pages: - Volume / Issue: 44 (6) Sequence Number: - Start / End Page: 2528 - 2537 Identifier: ISSN: 0305-1048