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  Subcortial cytoskeleton periodicity throughout the nervous system.

D'Este, E., Kamin, D., Velte, C., Göttfert, F., Simons, M., & Hell, S. W. (2016). Subcortial cytoskeleton periodicity throughout the nervous system. Scientific Reports, 6: 22741. doi:10.1038/srep22741.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-0B9F-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-0E45-2
Genre: Journal Article

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http://www.nature.com/articles/srep22741 (Publisher version)
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 Creators:
D'Este, E.1, Author              
Kamin, D.1, Author              
Velte, C., Author
Göttfert, F.1, Author              
Simons, M., Author
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: Superresolution fluorescence microscopy recently revealed a ~190 nm periodic cytoskeleton lattice consisting of actin, spectrin, and other proteins underneath the membrane of cultured hippocampal neurons. Whether the periodic cytoskeleton lattice is a structural feature of all neurons and how it is modified when axons are ensheathed by myelin forming glial cells is not known. Here, STED nanoscopy is used to demonstrate that this structure is a commonplace of virtually all neuron types in vitro. To check how the subcortical meshwork is modified during myelination, we studied sciatic nerve fibers from adult mice. Periodicity of both actin and spectrin was uncovered at the internodes, indicating no substantial differences between unmyelinated and myelinated axons. Remarkably, the actin/spectrin pattern was also detected in glial cells such as cultured oligodendrocyte precursor cells. Altogether our work shows that the periodic subcortical cytoskeletal meshwork is a fundamental characteristic of cells in the nervous system and is not a distinctive feature of neurons, as previously thought.

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Language(s): eng - English
 Dates: 2016-03-07
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/srep22741
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Title: Scientific Reports
Source Genre: Journal
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Pages: 8 Volume / Issue: 6 Sequence Number: 22741 Start / End Page: - Identifier: -