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  Spatiotemporal dynamics of sensory responses in layer 2/3 of rat barrel cortex measured in vivo by voltage-sensitive dye imaging combined with whole-cell voltage recordings and neuron reconstructions

Petersen, C. C. H., Grinvald, A., & Sakmann, B. (2003). Spatiotemporal dynamics of sensory responses in layer 2/3 of rat barrel cortex measured in vivo by voltage-sensitive dye imaging combined with whole-cell voltage recordings and neuron reconstructions. The Journal of Neuroscience: the Official Journal of the Society for Neuroscience, 23(4), 1298-1309. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12598618.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-0DFA-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-0DFB-0
Genre: Journal Article
Alternative Title : Spatiotemporal dynamics of sensory responses in layer 2/3 of rat barrel cortex measured in vivo by voltage-sensitive dye imaging combined with whole-cell voltage recordings and neuron reconstructions

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Petersen, Carl C. H.1, Author              
Grinvald, Amiram1, Author              
Sakmann, Bert1, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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Free keywords: barrel cortex; layer 2/3; voltage-sensitive dye; imaging; in vivo; sensory response
 Abstract: The spatiotemporal dynamics of the sensory response in layer 2/3 of primary somatosensory cortex evoked by a single brief whisker deflection was investigated by simultaneous voltage−sensitive dye (VSD) imaging and whole−cell (WC) voltage recordings in the anesthetized rat combined with reconstructions of dendritic and axonal arbors of L2/3 pyramids. Single and dual WC recordings from pyramidal cells indicated a strong correlation between the local VSD population response and the simultaneously measured subthreshold postsynaptic potential changes in both amplitude and time course. The earliest VSD response was detected 10−12 msec after whisker deflection centered above the barrel isomorphic to the stimulated principal whisker. It was restricted horizontally to the size of a single barrel−column coextensive with the dendritic arbor of barrel−column−related pyramids in L2/3. The horizontal spread of excitation remained confined to a single barrel−column with weak whisker deflection. With intermediate deflections, excitation spread into adjacent barrel−columns, propagating twofold more rapidly along the rows of the barrel field than across the arcs, consistent with the preferred axonal arborizations in L2/3 of reconstructed pyramidal neurons. Finally, larger whisker deflections evoked excitation spreading over the entire barrel field within ˜50 msec before subsiding over the next ˜250 msec. Thus the subthreshold cortical map representing a whisker deflection is dynamic on the millisecond time scale and strongly depends on stimulus strength. The sequential spatiotemporal activation of the excitatory neuronal network in L2/3 by a simple sensory stimulus can thus be accounted for primarily by the columnar restriction of L4 to L2/3 excitatory connections and the axonal field of barrel−related pyramids

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Language(s): eng - English
 Dates: 2002-11-272002-10-242002-12-022003-02-15
 Publication Status: Published in print
 Pages: 12
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 Table of Contents: -
 Rev. Type: Peer
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Title: The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
  Other : J. Neurosci.
Source Genre: Journal
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Publ. Info: Baltimore, MD : The Society
Pages: - Volume / Issue: 23 (4) Sequence Number: - Start / End Page: 1298 - 1309 Identifier: ISSN: 0270-6474
CoNE: https://pure.mpg.de/cone/journals/resource/954925502187_1