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  Motility, chemotaxis and aerotaxis contribute to competitiveness during bacterial pellicle biofilm development

Hölscher, T., Bartels, B., Lin, Y.-C., Gallegos-Monterrosa, R., Price-Whelan, A., Kolter, R., et al. (2015). Motility, chemotaxis and aerotaxis contribute to competitiveness during bacterial pellicle biofilm development. Journal of Molecular Biology (London), 427(23), 3695-3708. doi:10.1016/j.jmb.2015.06.014.

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IMPRS080s1.pdf (Supplementary material), 646KB
 
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Hölscher, Theresa1, Author           
Bartels, Benjamin2, Author           
Lin, Yu-Cheng, Author
Gallegos-Monterrosa, Ramses, Author
Price-Whelan, Alexa, Author
Kolter, Roberto, Author
Dietrich, Lars E. P., Author
Kovacs, Akos T., Author
Affiliations:
1IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, ou_421900              
2Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society, ou_421899              

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 Abstract: Biofilm formation is a complex process involving various signaling pathways and changes in gene expression. Many of the sensory mechanisms and regulatory cascades involved have been defined for biofilms formed by diverse organisms attached to solid surfaces. By comparison, our knowledge on the basic mechanisms underlying the formation of biofilms at air liquid interfaces, that is, pellicles, is much less complete. In particular, the roles of flagella have been studied in multiple solid-surface biofilm models but remain largely undefined for pellicles. In this work, we characterize the contributions of flagellum-based motility, chemotaxis and oxygen sensing to pellicle formation in the Gram-positive Bacillus subtilis. We confirm that flagellumbased motility is involved in, but is not absolutely essential for, B. subtilis pellicle formation. Further, we show that flagellum-based motility, chemotaxis and oxygen sensing are important for successful competition during B. subtilis pellicle formation. We report that flagellum-based motility similarly contributes to pellicle formation and fitness in competition assays in the Gram-negative Pseudomonas aeruginosa. Time-lapse imaging of static liquid cultures demonstrates that, in both B. subtilis and P. aeruginosa, a turbulent flow forms in the tube and a zone of clearing appears below the air liquid interface just before the formation of the pellicle but only in strains that have flagella. (C) 2015 Elsevier Ltd. All rights reserved.

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 Dates: 2015-11-20
 Publication Status: Published in print
 Pages: 14
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 Identifiers: DOI: 10.1016/j.jmb.2015.06.014
Other: IMPRS080
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Title: Journal of Molecular Biology (London)
  Other : J Mol Biol
Source Genre: Journal
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Publ. Info: London : Academic Press
Pages: - Volume / Issue: 427 (23) Sequence Number: - Start / End Page: 3695 - 3708 Identifier: ISSN: 0022-2836
CoNE: https://pure.mpg.de/cone/journals/resource/954922646042