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Schlagwörter:
Lasers and laser optics : Optical amplifiers; Medical optics and biotechnology : Turbid media; Fluorescence microscopy; Microscopy : Scanning microscopy; Microscopy : Three-dimensional microscopy; Nonlinear optics : Multiphoton processes
Zusammenfassung:
It is shown that two-photon fluorescence images can be obtained throughout almost the entire gray matter of the mouse neocortex by using optically amplified femtosecond pulses. The achieved imaging depth approaches the theoretical limit set by excitation of out-of-focus fluorescence.
