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  In Situ Cryo-Electron Tomography: A Post-Reductionist Approach to Structural Biology

Asano, S., Engel, B. D., & Baumeister, W. (2016). In Situ Cryo-Electron Tomography: A Post-Reductionist Approach to Structural Biology. Journal of Molecular Biology (London), 428(2, Part A. Special Issue: Study of biomolecules and biological systems: Proteins), 332-343. doi:10.1016/j.jmb.2015.09.030.

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 Urheber:
Asano, Shoh1, Autor           
Engel, Benjamin D.1, Autor           
Baumeister, Wolfgang1, Autor           
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Schlagwörter: TRANSMISSION ELECTRON-MICROSCOPY; PARTICLE CRYO-EM; 26S PROTEASOME; RADIATION-DAMAGE; SAMPLE PREPARATION; VITREOUS SECTIONS; INTACT-CELLS; MOLECULAR ARCHITECTURE; ANGSTROM RESOLUTION; SPATIAL PRECISIONcryo-EM; tomography; template matching; visual proteomics; subtomogram averaging;
 Zusammenfassung: Cryo-electron tomography is a powerful technique that can faithfully image the native cellular environment at nanometer resolution. Unlike many other imaging approaches, cryo-electron tomography provides a label-free method of detecting biological structures, relying on the intrinsic contrast of frozen cellular material for direct identification of macromolecules. Recent advances in sample preparation, detector technology, and phase plate imaging have enabled the structural characterization of protein complexes within intact cells. Here, we review these technical developments and outline a detailed computational workflow for in situ structural analysis. Two recent studies are described to illustrate how this workflow can be adapted to examine both known and unknown cellular complexes. The stage is now set to realize the promise of visual proteomics a complete structural description of the cell's native molecular landscape. (C) 2015 Elsevier Ltd. All rights reserved.

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Sprache(n): eng - English
 Datum: 2016
 Publikationsstatus: Erschienen
 Seiten: 12
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000370833000009
DOI: 10.1016/j.jmb.2015.09.030
 Art des Abschluß: -

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Projektname : ERC-2012-SyG_318987-ToPAG
Grant ID : 318987
Förderprogramm : -
Förderorganisation : European Commission

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Titel: Journal of Molecular Biology (London)
  Andere : J Mol Biol
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: London : Academic Press
Seiten: - Band / Heft: 428 (2, Part A. Special Issue: Study of biomolecules and biological systems: Proteins) Artikelnummer: - Start- / Endseite: 332 - 343 Identifikator: ISSN: 0022-2836
CoNE: https://pure.mpg.de/cone/journals/resource/954922646042