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  Removing Contamination-Induced Reconstruction Artifacts from Cryo-electron Tomograms

Fernandez, J.-J., Laugks, U., Schaffer, M., Bäuerlein, F. J. B., Khoshouei, M., Baumeister, W., et al. (2016). Removing Contamination-Induced Reconstruction Artifacts from Cryo-electron Tomograms. BIOPHYSICAL JOURNAL, 110(4), 850-859. doi:10.1016/j.bpj.2015.10.043.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-267F-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-2680-B
Genre: Journal Article

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 Creators:
Fernandez, Jose-Jesus1, Author
Laugks, Ulrike2, Author              
Schaffer, Miroslava2, Author              
Bäuerlein, Felix J. B.2, Author              
Khoshouei, Maryam2, Author              
Baumeister, Wolfgang2, Author              
Lucic, Vladan2, Author              
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: ELECTRON-MICROSCOPY; NOISE-REDUCTION; PHASE PLATE; SEGMENTATION; SPECIMENS; DIFFUSION; NEURONS; FILTER; CELLS
 Abstract: Imaging of fully hydrated, vitrified biological samples by electron tomography yields structural information about cellular protein complexes in situ. Here we present a computational procedure that removes artifacts of three-dimensional reconstruction caused by contamination present in samples during imaging by electron microscopy. Applying the procedure to phantom data and electron tomograms of cellular samples significantly improved the resolution and the interpretability of tomograms. Artifacts caused by surface contamination associated with thinning by focused ion beam, as well as those arising from gold fiducial markers and from common, lower contrast contamination, could be removed. Our procedure is widely applicable and is especially suited for applications that strive to reach a higher resolution and involve the use of recently developed, state-of-the-art instrumentation.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: ISI: 000370763800013
DOI: 10.1016/j.bpj.2015.10.043
 Degree: -

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Title: BIOPHYSICAL JOURNAL
Source Genre: Journal
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Publ. Info: 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 110 (4) Sequence Number: - Start / End Page: 850 - 859 Identifier: ISSN: 0006-3495