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  Visualizing the molecular sociology at the HeLa cell nuclear periphery

Mahamid, J., Pfeffer, S., Schaffer, M., Villa, E., Danev, R., Cuellar, L. K., et al. (2016). Visualizing the molecular sociology at the HeLa cell nuclear periphery. SCIENCE, 351(6276), 969-972. doi:10.1126/science.aad8857.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-2AE8-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-2AE9-0
Genre: Journal Article

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 Creators:
Mahamid, Julia1, Author              
Pfeffer, Stefan1, Author              
Schaffer, Miroslava1, Author              
Villa, Elizabeth1, Author              
Danev, Radostin1, Author              
Cuellar, Luis Kuhn1, Author              
Förster, Friedrich1, 2, Author              
Hyman, Anthony A.3, Author
Plitzko, Jürgen M.1, Author              
Baumeister, Wolfgang1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2Förster, Friedrich / Modeling of Protein Complexes, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565148              
3external, ou_persistent22              

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Free keywords: ATOMIC-FORCE MICROSCOPY; PORE COMPLEX; ELECTRON-MICROSCOPY; CRYOELECTRON MICROSCOPY; INTERMEDIATE-FILAMENTS; MECHANICAL-PROPERTIES; STRUCTURAL-ANALYSIS; PHASE PLATE; LAMIN; ARRANGEMENT
 Abstract: The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo-electron tomography (cryo-ET) to produce three-dimensional snapshots of the HeLa cell nuclear periphery. Subtomogram averaging and classification of ribosomes revealed the native structure and organization of the cytoplasmic translation machinery. Analysis of a large dynamic structure-the nuclear pore complex-revealed variations detectable at the level of individual complexes. Cryo-ET was used to visualize previously elusive structures, such as nucleosome chains and the filaments of the nuclear lamina, in situ. Elucidation of the lamina structure provides insight into its contribution to metazoan nuclear stiffness.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: ISI: 000370821400040
DOI: 10.1126/science.aad8857
 Degree: -

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Title: SCIENCE
Source Genre: Journal
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Publ. Info: 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA : AMER ASSOC ADVANCEMENT SCIENCE
Pages: - Volume / Issue: 351 (6276) Sequence Number: - Start / End Page: 969 - 972 Identifier: ISSN: 0036-8075