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  Control of Smc Coiled Coil Architecture by the ATPase Heads Facilitates Targeting to Chromosomal ParB/parS and Release onto Flanking DNA

Minnen, A., Bürmann, F., Wilhelm, L., Anchimiuk, A., Diebold-Durand, M.-L., & Gruber, S. (2016). Control of Smc Coiled Coil Architecture by the ATPase Heads Facilitates Targeting to Chromosomal ParB/parS and Release onto Flanking DNA. CELL REPORTS, 14(8), 2003-2016. doi:10.1016/j.celrep.2016.01.066.

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 Creators:
Minnen, Anita1, Author              
Bürmann, Frank1, Author              
Wilhelm, Larissa1, Author              
Anchimiuk, Ania1, Author              
Diebold-Durand, Marie-Laure1, Author              
Gruber, Stephan1, Author              
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1Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565151              

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Free keywords: BACILLUS-SUBTILIS SMC; STRUCTURAL MAINTENANCE; SUBCELLULAR-LOCALIZATION; BACTERIAL CHROMOSOME; MOLECULAR-BASIS; B. SUBTILIS; CONDENSIN; SEGREGATION; PROTEINS; ACTIVATION
 Abstract: Smc/ScpAB promotes chromosome segregation in prokaryotes, presumably by compacting and resolving nascent sister chromosomes. The underlying mechanisms, however, are poorly understood. Here, we investigate the role of the Smc ATPase activity in the recruitment of Smc/ScpAB to the Bacillus subtilis chromosome. We demonstrate that targeting of Smc/ScpAB to ParB/parS loading sites is strictly dependent on engagement of Smc head domains and relies on an open organization of the Smc coiled coils. We find that dimerization of the Smc hinge domain stabilizes closed Smc rods and hinders head engagement as well as chromosomal targeting. Conversely, the ScpAB sub-complex promotes head engagement and Smc rod opening and thereby facilitates recruitment of Smc to parS sites. Upon ATP hydrolysis, Smc/ScpAB is released from loading sites and relocates within the chromosome-presumably through translocation along DNA double helices. Our findings define an intermediate state in the process of chromosome organization by Smc.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: CELL REPORTS
Source Genre: Journal
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Publ. Info: 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 14 (8) Sequence Number: - Start / End Page: 2003 - 2016 Identifier: ISSN: 2211-1247