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  Nucleotide-dependent conformational changes of the AAA+ ATPase p97 revisited

Schuller, J. M., Beck, F., Lossl, P., Heck, A. J. R., & Förster, F. (2016). Nucleotide-dependent conformational changes of the AAA+ ATPase p97 revisited. FEBS LETTERS, 590(5), 595-604. doi:10.1002/1873-3468.12091.

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 Creators:
Schuller, Jan M.1, Author           
Beck, Florian2, Author           
Lossl, Philip3, Author
Heck, Albert J. R.3, Author
Förster, Friedrich1, Author           
Affiliations:
1Förster, Friedrich / Modeling of Protein Complexes, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565148              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
3external, ou_persistent22              

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Free keywords: VALOSIN-CONTAINING PROTEIN; MASS ANALYSIS; CRYO-EM; P97/VCP; REVEALS; COMMUNICATION; ASSEMBLIES; PROTEASOME; MICROSCOPY; RESOLUTIONAAA-ATPase; Cdc48; Cryo-EM; ERAD; protein quality control;
 Abstract: The ubiquitous AAA-ATPase p97 segregates ubiquitylated proteins from their molecular environment. Previous studies of the nucleotide-dependent conformational changes of p97 were inconclusive. Here, we determined its structure in the presence of ADP, AMP-PNP, or ATP-gamma S at 6.1-7.4 angstrom resolution using single particle cryo-electron microscopy. Both AAA domains, D1 and D2, assemble into essentially six-fold symmetrical rings. The pore of the D1-ring remains essentially closed under all nucleotide conditions, whereas the D2-ring shows an iris-like opening for ADP. The largest conformational changes of p97 are 'swinging motions' of the N-terminal domains, which may enable segregation of ubiquitylated substrates from their environment.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000371402700001
DOI: 10.1002/1873-3468.12091
 Degree: -

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Title: FEBS LETTERS
Source Genre: Journal
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Publ. Info: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL
Pages: - Volume / Issue: 590 (5) Sequence Number: - Start / End Page: 595 - 604 Identifier: ISSN: 0014-5793