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  Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation

Han, M., Zickler, L., Giese, G., Walter, M., Loesel, F. H., & Bille, J. F. (2004). Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation. Journal of Biomedical Optics, 9(4), 760-766. doi:10.1117/1.1756919.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-1961-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-1967-E
Genre: Journal Article
Alternative Title : Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation

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JBiomedicalOpt_9_2004_760.pdf (Any fulltext), 567KB
 
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 Creators:
Han, Meng, Author
Zickler, Leander, Author
Giese, Günter1, Author              
Walter, Matthias, Author
Loesel, Frieder H., Author
Bille, Josef F., Author
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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Free keywords: second harmonics; imaging; multiphoton; microscopy; cornea; femtosecond; all-solid-state laser
 Abstract: Nonlinear laser scanning microscopy is widely used for noninvasive imaging in cell biology and tissue physiology. However, multiphoton fluorescence imaging of dense, transparent connective tissue (e.g., cornea) is challenging since sophisticated labeling or slicing is necessary. High-resolution, high-contrast second harmonic generation (SHG) imaging of corneal tissue based on the intrinsic structure of collagen is discussed. The three-dimensional corneal ultrastructure in depths up to hundreds of microns can be probed noninvasively, without any staining or mechanical slicing. As an important application of second harmonic imaging in ophthalmology, the modification of corneal ultrastructure using femtosecond laser intrastromal ablation is systematically investigated to evaluate next-generation refractive surgical approaches.

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Language(s): eng - English
 Dates: 2003-10-242003-07-172003-11-122004-07-122004-07-01
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Journal of Biomedical Optics
Source Genre: Journal
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Publ. Info: Bellingham, WA : Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society
Pages: - Volume / Issue: 9 (4) Sequence Number: - Start / End Page: 760 - 766 Identifier: ISSN: 1083-3668
CoNE: https://pure.mpg.de/cone/journals/resource/954925607859