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  A genome-wide resource for the analysis of protein localisation in Drosophila

Sarov, M., Barz, C., Jambor, H., Hein, M. Y., Schmied, C., Suchold, D., et al. (2016). A genome-wide resource for the analysis of protein localisation in Drosophila. eLife, 5: e12068. doi:10.7554/eLife.12068.

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 Creators:
Sarov, Mihail, Author
Barz, Christiane1, Author              
Jambor, Helena, Author
Hein, Marco Y.2, Author              
Schmied, Christopher, Author
Suchold, Dana, Author
Stender, Bettina1, Author              
Janosch, Stephan, Author
Vikas, Vinay K.J., Author
Krisnan, R.T., Author
Aishwarya, K., Author
Ferreira, Irene R. S.1, Author              
Ejsmont, Radoslaw K., Author
Finkl, Katja1, Author              
Hasse, Susanne, Author
Kaempfer, Philipp, Author
Plewka, Nicole1, Author              
Vinis, Elisabeth, Author
Schloissnig, Siegfried, Author
Knust, Elisabeth, Author
Hartenstein, Volker, AuthorMann, Matthias2, Author              Ramaswami, Mani, AuthorVijayRaghavan, K., AuthorTomancak, Pavel, AuthorSchnorrer, Frank1, Author               more..
Affiliations:
1Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565168              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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 Abstract: The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.

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Language(s): eng - English
 Dates: 2016-02-20
 Publication Status: Published online
 Pages: 38
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.7554/eLife.12068
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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 5 Sequence Number: e12068 Start / End Page: - Identifier: Other: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X